a. What is the important of subculture in animal cell culture? And explain how do yo do subculture? b. Why we use these things in animal cell culture? Serum PBS DMEM Different well plates Different volume flasks
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- A subculture is a a. colony growing beneath the media surface b. culture made from a contaminant c. culture made in an embryo d. culture made from an isolated colonyMacConkey Agar is considered as: a. Differential CM only b. Enriched CM only c. Selective CM only d. Selective and differential CMThe factors for the manipulation of culture medium? pls answer is in short
- In not more than five lines, outline a method for preparation of tomato cell suspension culture and subculture.MacConkey agar plates represent ________ medium. a differential a minimal a selective both a differential and a selective both a minimal and a selectiveDescribe characteristics of Proteus Vulgaris in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar
- Explain shortly the concept of differential centrifugation and how you would limit pellet contamination which is a disadvantage of this technique.The thermal death point is best described as:(a) The highest temperature that will support growth of amicrobial culture(b) The length of time required for killing 100% of mi-crobes in a 24-hour culture that is heated to 100°C(c) The temperature at which a previously growing 24-hourculture will begin to die(d) The temperature at which 100% of the microbes in a24-hour culture will be killed in 10 minutesA microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…
- Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient AgarA culture initially has P0 number of bacteria. At t = 1 hour the number of bacteria is measured to be 3/2P0. If the rate of growth is proportional to the number of bacteria P(t) present at time t,determine the time necessary for the number of bacteria to triple.1. What are the colony shape, margin and elevation of colony of nutritient agar exposed in these types of exposure A. Soil B. Water C. Plant leaves D. Air
