1.724 1.708 IA

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter14: Biotechnology And Society
Section: Chapter Questions
Problem 12QP: DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that...
icon
Related questions
Topic Video
Question
FIGURE
1.724 1.708
10 20 30 40 50 60 70 80
10 20 30 40 50 60 70 80
FRACTIONS
RADIOACTIVITY
Transcribed Image Text:FIGURE 1.724 1.708 10 20 30 40 50 60 70 80 10 20 30 40 50 60 70 80 FRACTIONS RADIOACTIVITY
Equilibrium Density Gradient Centrifugation of
DNA Samples
Review the following terms using the internet before working on the problem:
radioactive labeling, DNA, cesium chloride, equilibrium centrifugation, DNA denaturation
using heat
EΧPERIΜΕT
Radioactively labeled bacterial DNA was analyzed by cesium chloride (CSCI) equilibrium
density gradient centrifugation. The radioactivity of fractions collected from the gradients,
and the densities of the two peak fractions, were determined. Graph A: Native DNA sample;
Graph B: DNA sample heated to 100°C and rapidly cooled on ice before centrifugation.
Transcribed Image Text:Equilibrium Density Gradient Centrifugation of DNA Samples Review the following terms using the internet before working on the problem: radioactive labeling, DNA, cesium chloride, equilibrium centrifugation, DNA denaturation using heat EΧPERIΜΕT Radioactively labeled bacterial DNA was analyzed by cesium chloride (CSCI) equilibrium density gradient centrifugation. The radioactivity of fractions collected from the gradients, and the densities of the two peak fractions, were determined. Graph A: Native DNA sample; Graph B: DNA sample heated to 100°C and rapidly cooled on ice before centrifugation.
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Patterns of inheritance
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co…
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning