Whether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?
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Whether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?
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- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.The last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet. Why do we need to wash the DNA using ice-cold ethyl alcohol? And why do we have to dry the DNA pellet?During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricity
- A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than one with 2,500 bp.True or false?In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is the purpose of this step? In DNA isolation from blood samples, why does the vial for blood storage contain EDTA? In the preparation for DNA isolation in plants, the plant source is refrigerated and ground prior to extraction. Why is this so? Why are DNA pellets air-dried before resuspension in buffer?A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2% agarose gel instead, what would you expect would happen to the migration rate of DNA and why?
- What would spread (fragmented) bands in the electrophoresis gel tell you about the quality of your DNA? Explain briefly, yet clearlyHow would the appearance of your DNA gel change if a 0.1% agarose gel slab was used? What about a 7% agarose gel slab? For separating small fragments of DNA, is it better to use a 7% gel or a 0.1% gel? Why?The following gel was produced in manual DNA sequencing. What would the unknown DNA template be that this gel represents?
- what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp DNA fragment and why?Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the process of extracting the DNA.How does what you see on the acrylamide gel reflect what is presented on a sequencing chromatograph?