1.Antibiotics that are active against G= and G- organisms such as Blank 1, Blank 2, and Blank 3 contain both proteinogenic and non- proteinogenic amino acids. 2.In the structure of vancomycin, the Blank 1 moiety contributes to its solubility in polar medium
Q: 4. Liver alcohol dehydrogenase (LADH) catalyzes a reversible, pH-dependent oxidation of an alcohol…
A: Density=massvolumeDensity of methanol=mass of methanolvolume of methanol0.79 g/ml=mass of…
Q: 1. Why a peptide bond can't rotate freely? 6%. 2. The and are two amino acids usually will break an…
A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since…
Q: Diagram to compare & contrast CATABOLISM vs ANABOLISM such as its process/mechanism, relation with…
A: Anabolism and Catabolism are the two broad type of biochemical reaction in metabolism where…
Q: The BSA precursor protein is 607 amino acids long and has a molecular weight 69.324 kDa. If a…
A: BSA is globular proteins. 1kDa = 1.66 * 10-21 gm The molecular weight of BSA precursor protein is…
Q: What is the direction of transcription in the plasmid shown below? Explain your answer based on the…
A: Transcription is the process of synthesis of RNA using DNA as the template and is catalyzed by
Q: Maintaining blood glucose levels above 40 mg/dL is important so that all cells are able to take in…
A: Glycogen is a polymer with a branching structure. Carbohydrates are stored in this form in the human…
Q: Give the 3 major pathways that eventually become entry points of molecules into the Krebs Cycle.…
A: The Krebs cycle, or citric acid cycle, occurs in the mitochondrion of a cell. The citric acid cycle…
Q: Why aren’t your cells poisoned to death even though they create toxins like hydrogen peroxide?
A: The hydrogen peroxide formed produced during normal cellular metabolism. It is a potent toxin that…
Q: What is the detection principle of iodine test for starch
A: The iodine test is a quantitative analysis of carbohydrates to distinguish polysaccharides from…
Q: Fill in the blanks in the table below regarding the similarities and differences between two…
A: The replication of DNA involves opening up a double-stranded DNA molecule and creating a…
Q: Given Sorbitol, Briefly explain its expected reaction (based on their structural formula) to the…
A: Sorbitol is a carbohydrate that is classified as a sugar alcohol or polyol.
Q: In cholesterol, to which ring of the steroid system is the hydroxyl group attached? B O A
A: cholesterol is a sterol and is the primary compound from which synthesis of many steroid hormones,…
Q: 2. ( To the right is a schematic diagram of His the active site in the Michaelis complex of a-chy-…
A: Chymotrypsin is a protease that cleaves a peptide at the C-terminal of all aromatic amino acid.…
Q: Given Raffinose, Briefly explain its expected reaction (based on their structural formula) to the…
A: Raffinose is a trisacharide made by three monosacharide units galactose Glucose and fructose.…
Q: Match lipid structures in column A with its lipid type in column B
A: Lipids are the group of organic components having oily or greasy consistency. Lipids are a group of…
Q: 6. When a concentrated alkali solution acts on the purine cycle, it breaks down: A. Ester group B.…
A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since…
Q: Please explain what happened in the reaction. How did H2SO4 and 3H2O reacted with the glucose?
A: Carbohydrates are polyhydroxy aldehydes or ketones or compounds that yield them on hydrolysis.…
Q: How should you prepare your running buffer?
A: SDS-PAGE is method of separation of protein based on its size. Running buffer of the SDS-PAGE…
Q: Which of the following glycerophospholipid has a phosphate ester attached to a sugar moiety O…
A: Phosphoglycerides or Glycerophospholipids are the main component of biological membrane, usually…
Q: Match the following biogeochemical cycles terms to the correct definiton. In this cycle the…
A: Introduction: The term biogeochemical cycle is a natural process that recycles nutrients from the…
Q: Provide a simple sketch of the covalent intermediate likely to form between active site serine…
A: DPCC: Diphenylcarbamyl chloride has two phenyl group attached to carbamyl chloride. Serine Protease:…
Q: Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest…
A: DEAE-cellulose contains an diethylaminoethyl group and is positively charged at neutral pH.
Q: In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Solid…
A: During replication forks, Okazaki fragments are transient components of lagging strand DNA…
Q: At which position(s) are amino acids limited to those Which amino acid(s) are more commonly found at…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Question 4 Match the each enzyme deficiency with their corresponding disease B-hexosaminidase A A.…
A: Enzyme deficiency results in certain metabolic disorders and results in serious diseases.
Q: is used in gluconeogenesis that bypasses step 1 of Glycolysis in the production of free glucose from…
A: Gluconeogenesis is a process that transform non-carbohydrate substrate into glucose.the principal…
Q: Proteins called molecular chaperones assist in the process of protein folding. One class of…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Match the best possible fit for the following Animal virus replication stages. A. Attachment B.…
A: Viruses contain nucleic acid/ribonucleic acid in the core region while the exterior/…
Q: Which of the following is NOT a primary method used to regulate the activity of cyclin-dependent…
A: Cyclin-dependent kinases (CDKs) are the protein kinases which plays a role in regulating the cell…
Q: In a double-stranded DNA molecule, how are the sequences of each strand related to each other? A…
A: DNA are the nucleotide which contains genetic information in our body and are found in nucleus.
Q: Given ohationg im which is a Jlist of orgamelles ņ mitochondira, endopla smic rediculerm,pesoxisomes…
A: A cell is composed of a cell membrane, cytoplasm, and cellular organelles in the cytoplasm.…
Q: 7. What is the base sequence, specified in the 5' to 3' direction, for a segment of newly formed DNA…
A: The genetic material in most organism is double stranded DNA with the two strands running in…
Q: 5. Compare and constrast the energy content of fats, carbohydrates and proteins.
A: Carbohydrates, proteins and fats are three major form of biomolecules found in living beings. These…
Q: Please dont copy here in Bartleby • Distinguish between a purine, pyrimidine, nucleoside,…
A: DNA and RNA are made up of long chains of nucleotides. The nucleic acid polymer has…
Q: tochondria are considered powernou: eukaryotiC celis because they prd |cell can subsequentiy use as…
A: ATP are the energy currency of the cell. ATP helps the cell to sustain different activities. We…
Q: How histone and micro RNA controls gene expressi
A: Histones are positively charged basic proteins that associate with DNA in the nucleus and forms…
Q: Think about ONE possible consequence with a brief explanation if the primers were not removed after…
A: Introduction: DNA is a genetic material that defines every cell. Before a cell duplicates and is…
Q: जन्का ली देकर परार देेल लब्पsogene sn जाक ? A) Hn iso2gme cam be Coeed 0Siलह कसक्ट कर Te १riance…
A: Enzymes are the protein molecules that increase the rate of reactions by decreasing the activation…
Q: Calculate the free energy change for glucose entry into cells when the extracellular concentration…
A: Free energy change The change in free energy (ΔG) is basically the difference between the heat…
Q: Why are unsaturated fats considered healthier?
A: Unsaturated fatty acids consist of a double bond between molecules of the fatty acid chains, whereas…
Q: How many ATP molecules are produced when 6 molecules of glucose enter glycolysis?
A: Glycolysis is a catabolic pathway in which six Carbon Glucose is catabolised into two 2carbon…
Q: The picture below shows the body's response to acute stress, which is to release adrenaline…
A: Introduction: Adrenergic neurons release norepinephrine as the primary neurotransmitter. These…
Q: Identify the 4 steps of gluconeoegenesis that are different from glycoslysis. Write the reactants,…
A: Gluconeogenesis (GNG) is a metabolic pathway that results in the generation of glucose from certain…
Q: When human hemoglobin undergoes a mutation, the mutant protein usually does not replace all of the…
A: The cytosol of red blood cells contains the oxygen-carrying globular protein hemoglobin, which is…
Q: Review method used to increase the solubility of a drug under the following headings co solvents PH…
A: Bioavailability is a powerful determinant of drug absorption. It represents the administered dose…
Q: Nutrition Facts Calories 112 Total Fat 0g Total Carbohydrates 21 g Protein 7g What percentage of the…
A: Introduction: Calories are the amount of energy or heat that takes to raise the temperature of one…
Q: Examine which of the following statement are FALSE about enzymes. * (Please choose one correct…
A: Enzymes are substances that enhance the rate of chemical reaction and facilitate the formation of…
Q: How does compromised pyruvate kinase activity lead to anemia?
A: "Since you have posted multiple question we will answer the first question for you. If you want any…
Q: In plants, under what solution conditions are Calvin cycle and gluconeogenesis enzymes most active?…
A: Gluconeogenesis is the process of Synthesis of glucose from non Carbohydrate sources like aminoacids…
Q: The process of protein decomposition by pepsin enzymes in stomach is assumed and modeled as a batch…
A: The reaction kinetics given in the pepsin digestion reaction follows Michaelis-Menten Kinetics. The…
Step by step
Solved in 3 steps with 1 images
- TOPIC: Precipitation of Proteins by Alkaloidal Reagents 1. In what way can alkaloidal reagents precipitate proteins? ____________________________________________________________________ ____________________________________________________________________ 2. In excess of alkaloidal reagent, did the precipitate formed dissolve or not? What is the evidence for your answer? ____________________________________________________________________ ____________________________________________________________________PRECIPITATION OF PROTEINS 2. Give the rationale for the following: a) The use of picric acid in burns b) The use of tannic acid in diarrhea 2. List 5 agents (physical or chemical) which could be used to destroy the life of microorganisms by coagulating its protein.Rationalize the following observations.(a) Serine is the amino acid residue that can be replaced with theleast effect on protein structure and function.(b) Replacement of tryptophan causes the greatest effect on protein structure and function.(c) Replacements such as Lys -> Arg and Leu -> Ile usually havevery little effect on protein structure and function.
- PRECIPITATION OF PROTEINS What are peptides? Do all proteins possess peptide bonds? Do all proteins respond to Biuret’s test? What is the difference between coagulation and denaturation? Why is egg white used as an antidote for lead and mercurial poisoning? Explain its mechanism of action. 4.Why is silver nitrate used in the cauterization of wound? Explain your answer.15. Which of the following glycans can be released from proteins using ?β-elimination? a. Complex N-linked glycans b. Glycosaminoglycans c. Mucin type O-glycans d Hyaluronic acid 16. Which of the following glycans are made by the addition of sugars to the reducing end of a growing glycan chain? a. Hyaluronan b. Heparan sulfate c. Mucin type O-glycans d. Gangliosides e. CelluloseHelp please. MD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per milliliter of morphine SO4. How many milliliters of the stock solution is required to fill the Rx?
- Can we purify mCherry protein (His-tag attached) with immobilised metal ions affinity chromatography (Ni-IDA)? If so, how can we confirm whether the protein is purified and how to increase its purity?topic: Bradford AssayThere are numerous methods of protein determination in use, but this module focuses on the Bradford assay.The Bradford assay is a dye-binding method that employs Coomassie Brilliant Blue G-250, whose structureis shown in Figure 2.3.4.1. Coomassie Brilliant Blue G-250 is a dye that interacts with proteins throughhydrophobic and electrostatic interactions. What are the identities and functions of the components of the Bradford reagent in protein contentdetermination?Please explain!!! select all that are right the same Protein X was purified from tissue source A and tissue source B, yielding the following results: Protein X, sample A: specific activity = 100 U/mg; fold-purification = 200 Protein X, sample B: specific activity = 1000 U/mg; fold-purification = 100 Which of the following statements are correct? a) Protein X, sample A has a higher level of purity than Protein X, sample B. b) Protein X, sample B has a higher level of purity than Protein X, sample A. c) Tissue source A has a higher specific activity for Protein X than tissue source B. d) Tissue source B has a higher specific activity for Protein X than tissue source A.
- Wh are doing this procedures can you explain? (ex. heating or adding chemicals etc.) 1) Purification of Vitelline from Egg Yolk-Experimental ProcedureMeasure the volume of 3 egg yolks and mix by adding an equal volume of NaCl solution.Extract the mixture with 3-fold volumes of ether and separate the aqueous phase.Do the same procedure 3 times.Mix the sample with water and rinse.It is expected for the protein to collapse.Some more water is added in order to check whether the collapse occurs completely.The sample is centrifuged and the precipitate is dried. 2) Purification of Plasma Albumin and Fibrinogen-Experimental ProcedureAdd ammonium sulfate to 10 ml plasma up to 25% saturation.Separate the collapsed fibrinogen by centrifuge.Increase the saturation level to 33% by adding (NH4) 2SO4.Separate the globulin by centrifugation.Separate prodoglobulins by increasing the saturation level to 46%.Increase the saturation level up to 64% to precipitate albumin.Separate the albumin by centrifuge…16. The lipopolysaccharide of Gram-negative bacteria is composed of the following EXCEPT: Polysaccharide Lipoteichoic acid Phospholipid O-PolysaccharideA vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working