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1.knowing the Control is 3.11 x 10-11 moles.cm-2.sec-1 and the is CFTR-CRISPR: 3.11 x 10-10 .cm-2.sec-1. how did they get and explain how? that

1.knowing the Control is 3.11 x 10-11 moles.cm-2.sec-1 and the is CFTR-CRISPR: 3.11 x 10-10 .cm-2.sec-1. how did they get and explain how? that

Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...
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1.knowing the Control is 3.11 x 10-11 moles.cm-2.sec-1 and the is CFTR-CRISPR: 3.11 x 10-10 .cm-2.sec-1. how did they get and explain how? that
Calibri (Bo... BIUV I of 1 41 CONTROL CFTR- CRISPR 16 A A Aa A X₂ X² A 196 words 呕 You are employed in a gene therapy laboratory to test the effectiveness of a vector for correcting the AF508-CFTR mutation. The vector uses CRISPR-CAS9 genome editing to correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was performed using nasal airway cells obtained from a patient with the AF508 CFTR mutation which were transformed with either a control vector consisting of an inert CRISPR-CAS-9 construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR). The following transepithelial voltage values were obtained for the drug regimen shown under open circuit and current clamp (injected current of 10μA) conditions: V (mv) AVRt (mv) R (2.cm-2) (μA.cm-²) Vte (mv) AVRt (mv) R (S2.cm-²) (uA.cm-²) V C Baseline -78 8 800 98 -38 9 900 42 A/ Paragraph Styles Amiloride -2 16 1600 1 -6 27 2700 2 Focus #tv A Calculate the Molar Cl- Flux for both "CONTROL" and "CFTR-CRISPR". Avogadro's number is 6.022x1023 atoms.mole¹. Give your answer as moles.cm-2.sec-1 to two decimal places followed by power of 10 magnitude (eg 5.66 x10-10 would give answers of 5.66 and -10) E E Forskolin -4 7.5 O 2 750 5 -23 6 600 38 Comments E Dictate Sensitivity Inh172 -3 13 1300 2 -9 11 1100 8 Editir s
Transcribed Image Text:Calibri (Bo... BIUV I of 1 41 CONTROL CFTR- CRISPR 16 A A Aa A X₂ X² A 196 words 呕 You are employed in a gene therapy laboratory to test the effectiveness of a vector for correcting the AF508-CFTR mutation. The vector uses CRISPR-CAS9 genome editing to correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was performed using nasal airway cells obtained from a patient with the AF508 CFTR mutation which were transformed with either a control vector consisting of an inert CRISPR-CAS-9 construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR). The following transepithelial voltage values were obtained for the drug regimen shown under open circuit and current clamp (injected current of 10μA) conditions: V (mv) AVRt (mv) R (2.cm-2) (μA.cm-²) Vte (mv) AVRt (mv) R (S2.cm-²) (uA.cm-²) V C Baseline -78 8 800 98 -38 9 900 42 A/ Paragraph Styles Amiloride -2 16 1600 1 -6 27 2700 2 Focus #tv A Calculate the Molar Cl- Flux for both "CONTROL" and "CFTR-CRISPR". Avogadro's number is 6.022x1023 atoms.mole¹. Give your answer as moles.cm-2.sec-1 to two decimal places followed by power of 10 magnitude (eg 5.66 x10-10 would give answers of 5.66 and -10) E E Forskolin -4 7.5 O 2 750 5 -23 6 600 38 Comments E Dictate Sensitivity Inh172 -3 13 1300 2 -9 11 1100 8 Editir s
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