11. The free energy of the two biomolecules is higher when they are not interacting as compared to the free energy of the two biomolecules when they come together. 12. Changing a positive charge in one of the biomolecules to a negative charge could lead to a positive change in enthalpy, which would lead to a more negative change in free energy. 13. Addition of hydrogen bonding partners on the two biomolecules could lead to a more stable interaction and thus a more negative change in free energy. 14. The interaction between the two biomolecules is likely an entropically driven process. 15. As the stability of the interaction between the two biomolecules increases the free energy change for their binding becomes more exergonic.

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