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Tryptophan synthase is one of the enzymes synthesized from the trp mRNA. In wild-type E. coli, the trp mRNA has a short half-life, but the tryptophan synthase half-life is much longer. To investigate how changes to the stability of the enzyme or its mRNA affect enzyme activity, two strains of E. coli were engineered. Strain A stabilizes the trp mRNA and strain B rapidly degrades tryptophan synthase. The wild-type, A, and B strains were grown in a medium with glucose as the sole carbon source. After several generations, tryptophan was added to all three cultures and tryptophan synthase activity was measured periodically. Note: Evaluate each condition as a simple model, where changes in the stability of trp mRNA or tryptophan synthase do not elicit secondary effects in the cells. Select the statements that describe the expected change in tryptophan synthase activity after the addition of tryptophan. In strain B, since both the trp mRNA and tryptophan synthase are rapidly degraded, the enzyme activity will drastically decrease. O In strain A, the trp mRNA is stable but the presence of tryptophan in the medium will cause the degradation of tryptophan synthase and abruptly decrease enzyme activity. O In the wild-type strain, the trp mRNA is degraded rapidly. However, since the tryptophan synthase is stable, the enzyme activity per cell will remain unchanged as the cells divide. In the wild-type strain, trp mRNA is unstable and will be degraded rapidly. As the cells continue to divide, tryptophan synthase will be diluted out and enzyme activity per cell will decrease. O In strain A, the trp mRNA will not be degraded and the cells will continue to synthesize tryptophan synthase. However, as the cells divide, the amount of enzyme activity per cell will slowly decrease.

Tryptophan synthase is one of the enzymes synthesized from the trp mRNA. In wild-type E. coli, the trp mRNA has a short half-life, but the tryptophan synthase half-life is much longer. To investigate how changes to the stability of the enzyme or its mRNA affect enzyme activity, two strains of E. coli were engineered. Strain A stabilizes the trp mRNA and strain B rapidly degrades tryptophan synthase. The wild-type, A, and B strains were grown in a medium with glucose as the sole carbon source. After several generations, tryptophan was added to all three cultures and tryptophan synthase activity was measured periodically. Note: Evaluate each condition as a simple model, where changes in the stability of trp mRNA or tryptophan synthase do not elicit secondary effects in the cells. Select the statements that describe the expected change in tryptophan synthase activity after the addition of tryptophan. In strain B, since both the trp mRNA and tryptophan synthase are rapidly degraded, the enzyme activity will drastically decrease. O In strain A, the trp mRNA is stable but the presence of tryptophan in the medium will cause the degradation of tryptophan synthase and abruptly decrease enzyme activity. O In the wild-type strain, the trp mRNA is degraded rapidly. However, since the tryptophan synthase is stable, the enzyme activity per cell will remain unchanged as the cells divide. In the wild-type strain, trp mRNA is unstable and will be degraded rapidly. As the cells continue to divide, tryptophan synthase will be diluted out and enzyme activity per cell will decrease. O In strain A, the trp mRNA will not be degraded and the cells will continue to synthesize tryptophan synthase. However, as the cells divide, the amount of enzyme activity per cell will slowly decrease.

Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter9: Gene Expression And Gene Regulation
Section: Chapter Questions
Problem 10QP: The pre-mRNA transcript and protein made by several mutant genes were examined. The results are...
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Tryptophan synthase is one of the enzymes synthesized from the trp mRNA. In wild-type E. coli, the trp mRNA has a short half-life, but the tryptophan synthase half-life is much longer. To investigate how changes to the stability of the enzyme or its mRNA affect enzyme activity, two strains of E. coli were engineered. Strain A stabilizes the trp mRNA and strain B rapidly degrades tryptophan synthase. The wild-type, A, and B strains were grown in a medium with glucose as the sole carbon source. After several generations, tryptophan was added to all three cultures and tryptophan synthase activity was measured periodically. Note: Evaluate each condition as a simple model, where changes in the stability of trp mRNA or tryptophan synthase do not elicit secondary effects in the cells. Select the statements that describe the expected change in tryptophan synthase activity after the addition of tryptophan. In strain B, since both the trp mRNA and tryptophan synthase are rapidly degraded, the enzyme activity will drastically decrease. In strain A, the trp mRNA is stable but the presence of tryptophan in the medium will cause the degradation of tryptophan synthase and abruptly decrease enzyme activity. O In the wild-type strain, the trp mRNA is degraded rapidly. However, since the tryptophan synthase is stable, the enzyme activity per cell will remain unchanged as the cells divide. O In the wild-type strain, trp mRNA is unstable and will be degraded rapidly. As the cells continue to divide, tryptophan synthase will be diluted out and enzyme activity per cell will decrease. In strain A, the trp mRNA will not be degraded and the cells will continue to synthesize tryptophan synthase. However, as the cells divide, the amount of enzyme activity per cell will slowly decrease.
Transcribed Image Text:Tryptophan synthase is one of the enzymes synthesized from the trp mRNA. In wild-type E. coli, the trp mRNA has a short half-life, but the tryptophan synthase half-life is much longer. To investigate how changes to the stability of the enzyme or its mRNA affect enzyme activity, two strains of E. coli were engineered. Strain A stabilizes the trp mRNA and strain B rapidly degrades tryptophan synthase. The wild-type, A, and B strains were grown in a medium with glucose as the sole carbon source. After several generations, tryptophan was added to all three cultures and tryptophan synthase activity was measured periodically. Note: Evaluate each condition as a simple model, where changes in the stability of trp mRNA or tryptophan synthase do not elicit secondary effects in the cells. Select the statements that describe the expected change in tryptophan synthase activity after the addition of tryptophan. In strain B, since both the trp mRNA and tryptophan synthase are rapidly degraded, the enzyme activity will drastically decrease. In strain A, the trp mRNA is stable but the presence of tryptophan in the medium will cause the degradation of tryptophan synthase and abruptly decrease enzyme activity. O In the wild-type strain, the trp mRNA is degraded rapidly. However, since the tryptophan synthase is stable, the enzyme activity per cell will remain unchanged as the cells divide. O In the wild-type strain, trp mRNA is unstable and will be degraded rapidly. As the cells continue to divide, tryptophan synthase will be diluted out and enzyme activity per cell will decrease. In strain A, the trp mRNA will not be degraded and the cells will continue to synthesize tryptophan synthase. However, as the cells divide, the amount of enzyme activity per cell will slowly decrease.
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