12 The following results were obtained in an experiment to determine spermine by high performance thin layer chromatography of one of its fluorescent derivatives. 36 269 Fluorescence intensity Spermine, ng 69 184 235 301 327 18 30 45 60 75 90 Determine the best polynomial calibration curve through these points. (Based on Linares, R. M., Ayala, J. H., Afonso, A. M. and Gonzalez, V. 1998. Analyst 123: 725)

Appl Of Ms Excel In Analytical Chemistry
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ISBN:9781285686691
Author:Crouch
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Chapter14: Chromatography
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12 The following results were obtained in an experiment to determine spermine
by high performance thin layer chromatography of one of its fluorescent
derivatives.
Fluorescence intensity
Spermine, ng
36
69
184
235
269
301
327
6
18
30
45
60
75
90
Determine the best polynomial calibration curve through these points.
(Based on Linares, R. M., Ayala, J. H., Afonso, A. M. and Gonzalez, V. 1998.
Analyst 123: 725)
Transcribed Image Text:12 The following results were obtained in an experiment to determine spermine by high performance thin layer chromatography of one of its fluorescent derivatives. Fluorescence intensity Spermine, ng 36 69 184 235 269 301 327 6 18 30 45 60 75 90 Determine the best polynomial calibration curve through these points. (Based on Linares, R. M., Ayala, J. H., Afonso, A. M. and Gonzalez, V. 1998. Analyst 123: 725)
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