12 The following results were obtained in an experiment to determine spermine by high performance thin layer chromatography of one of its fluorescent derivatives. 36 269 Fluorescence intensity Spermine, ng 69 184 235 301 327 18 30 45 60 75 90 Determine the best polynomial calibration curve through these points. (Based on Linares, R. M., Ayala, J. H., Afonso, A. M. and Gonzalez, V. 1998. Analyst 123: 725)
12 The following results were obtained in an experiment to determine spermine by high performance thin layer chromatography of one of its fluorescent derivatives. 36 269 Fluorescence intensity Spermine, ng 69 184 235 301 327 18 30 45 60 75 90 Determine the best polynomial calibration curve through these points. (Based on Linares, R. M., Ayala, J. H., Afonso, A. M. and Gonzalez, V. 1998. Analyst 123: 725)
Chapter14: Chromatography
Section: Chapter Questions
Problem 6P
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