Mass spectrometry and UV/Vis absorbance spectroscopy can both be used as letectors for liquid chromatography experiments. a. In simple systems, quantifying the concentration of an analyte is usually easier with UV/Vis than with MS. Explain why.

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter33: High-performance Liquid Chromatography
Section: Chapter Questions
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Please answer B part only
6.
Mass spectrometry and UV/Vis absorbance spectroscopy can both be used as
detectors for liquid chromatography experiments.
a. In simple systems, quantifying the concentration of an analyte is usually easier
with UV/Vis than with MS. Explain why.
b. Because MS is more sensitive and more selective, we often use it for quantitative
analysis anyway. Explain how you would design a quantitative LC/MS
experiment.
Transcribed Image Text:6. Mass spectrometry and UV/Vis absorbance spectroscopy can both be used as detectors for liquid chromatography experiments. a. In simple systems, quantifying the concentration of an analyte is usually easier with UV/Vis than with MS. Explain why. b. Because MS is more sensitive and more selective, we often use it for quantitative analysis anyway. Explain how you would design a quantitative LC/MS experiment.
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