1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structures

Q: hello, can you draw the following on endospore staining in 1000x objective? thank you.   - 1 day…

A: Bacillus is a genus which is a endospore forming, rod shaped, gram positive bacteria.

Q: What are the main characteristics of scanning electron microscopy?

A: Electrons are the negatively charged particles having minus 1 charge  and represented by e- ,…

Q: What is agar and why is it necessary for growing bacteria on plates?

A: Agar is a mixture of two polysaccharides- agarose and agaropectin. It is derived from red-purple…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: How is xylol used to clean the lens of a microscope? What may happenif xylol is constantly used for…

A: The microscope can be defined as the laboratory instrument which is utilized to examine objects that…

Q: Would lysozyme or penicillin affect pneumoniae?

A: As per the guidelines, we are supposrd to answer only one question in case of multiple posted.…

Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…

A: The gram-positive and negative cells differ in their cell wall composition.

Q: Why is oil immersion necessary to see bacteria in the laboratory?

A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…

Q: Which of the microscope objectives is most satisfactory for studying bacteria? Why?

A: For studying bacteria , under the microscope , 100X objective oil immersion lens is generally used…

Q: Why are bacterial cells generally stained for microscopic viewing?

A: Staining bacterial cells prior to microscopic viewing is a routine process in most of microbiology…

Q: Why is it sometimes difficult to attain both good resolution and good contrast at the same time for…

A: Magnification and resolution are important because the magnification in larges the image and the…

Q: What are types of cell culture media?

A: A cell culture medium is solid or liquid in nature. It is a mixture of all necessary nutrients…

Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…

A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…

Q: Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of…

A: Cell staining is a technique that is used in microscopy where certain chemicals can stick to…

Q: What is the purpose of using a biological stain when microscopically examining cellular components?

A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…

Q: What is the difference between conventional optical microscopes and inverted microscopes?

A: Introduction Conventional optical microscopes:- This type of microscope uses visible light (400-700…

Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…

A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…

Q: Why must materials and instruments used in tissue culture be kept sterile?

A: Sterilization of explants is a key step in plant tissue culture.

Q: How is true microbial motility distinguished from other kinds of motion like Brownian movement that…

A: Q. How is true microbial motility distinguished from other kinds of motion like Brownian movement…

Q: Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3…

A: In contrary to negative staining, which leaves the bacteria unstained against a dark backdrop,…

Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: What is the difference between a scanning and a transmission electron microscope? When do you use…

A: Microscopes are the instruments that allow scientists to study objects that cannot be seen through…

Q: Why are chemical stains required for visualizing cells and tissues with the basic light microscope?

A: Cell staining is a technique that is used to better visualize cells and cell components under a…

Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…

A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…

Q: a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a…

A: The staining is a very important process in microbiology that is used to identify specific…

Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?

A: The appearance of bacteria, when stained with safranin, is to be determined.

Q: What should be considered when preparing a monolayer cell culture?

A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…

Q: why the Gram stain can differentiate between Gram positive and Gramnegative cells?

A: *Gram staining is a technique used to differentiate two groups of bacteria into gram positive and…

Q: Why is it important to center your object before moving to higher magnifications in a light…

A: A microscope is a crucial tool in the study of organisms that are very minute or to study the cells…

Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?

A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…

Q: Why do we use oil with microscope ?

A: ••In light microscopy, in order to increase the resolving power we use oil Immersion techniques

Q: What types of compounds make up the gels used in electrophoresis?

A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…

Q: You use the Gram staining procedure to stain a bacterium that lacks a cell wall. Would you consider…

A: The stirring process is very much important for visualisation and identification of any living…

Q: Which Two types of electron microscopy are in routine use in microbiology? Explain

A: The electron microscope is an instrument that uses the beam of electrons to examine objects on a…

Q: Principle/ technique used in Flagella Stain Method/ Preparation/ Procedure of Flagella Stain

A: Flagella are narrow, slender whip-like structures made by flagellin protein that emerges from the…

Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?

A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…

Q: Why is the resolution so superior in the electron microscope?

A: A beam of electrons that travel in wavelike patterns when accelerated to high speeds form the images…

Q: What is the formula used to describe the resolution of a microscope lens, and what are the…

A: The term resolution is used in microscopy to characterize a microscope's capacity to identify…

Q: How can colony morphology be used in the identification of bacterial species? What are the…

A: NOTE: AS PER OUR GUIDELINES, WE ARE ONLY SUPPOSED TO ANSWER THE FIRST THREE QUESTIONS, PLEASE…

Q: agnification is required to observe microorganisms?

A: A microorganism, often known as a microbe, is a microscopic organism that can be a single cell or a…

Q: Discuss the importance of why flagellar stains must be freshly prepared?

A: Flagella Some bacteria, archaea, and eukaryotes whihcb are usually stayed to define as the microbe…

Q: Why should freshly collected samples be used for microscopic examination?

A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…

Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…

A: The gram-positive and gram-negative bacteria can be differentiated based on their cell wall elements…

Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…

A:

Q: Why do acidic dyes not stain bacterial cells?

A: Stains may be divided into two classes based on the nature of their charged group Basic/ cationic…

Q: Why do we stain some organisms before viewing them under a microscope? (see top of p 14)

A: Microbiology is the study of microorganism. The organisms that cannot be seen through naked eyes are…

Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?

A: The human eye can see objects upto 0.1 millimeters. If the objects are smaller than this, the human…

Q: Image A: Image B: 25 um O A. image A- scanning electron microscope, Image B- phase contrast…

A: Scanning electron microscope (SEM): It produce the image of the surface area by scanning with the…

Q: What is the function of staining in light microscopy?

A: The light microscopy or light microscope represents a laboratory instrument required for the…