1、You have a 250 bp DNA sequence from a human protein-coding gene. What's the best tool to find out which gene matches this sequence? BLAST A Clustal algorithm (such as Clustal Omega) A Burrows-Wheeler algorithm (such as BWA) Just Google it 2、Sequence alignment algorithms like Burrows-Wheeler were primarily designed to handle many (millions) of short sequences and match them to a reference genome efficiently. Another property of these algorithms makes them suitable for use with RNAseq data. What property is this? Burrows-Wheeler can distinguish which strand of reference genome matches a sequencing read. Burrows-Wheeler can align a sequencing read that matches two different exons. Burrows-Wheeler uses a pre-compiled and compressed index of the reference genome. Burrows-Wheeler can be parallelized.
1、You have a 250 bp DNA sequence from a human protein-coding gene. What's the best tool to find out which gene matches this sequence?
BLAST
A Clustal algorithm (such as Clustal Omega)
A Burrows-Wheeler algorithm (such as BWA)
Just Google it
2、Sequence alignment algorithms like Burrows-Wheeler were primarily designed to handle many (millions) of short sequences and match them to a reference genome efficiently. Another property of these algorithms makes them suitable for use with RNAseq data. What property is this?
Burrows-Wheeler can distinguish which strand of reference genome matches a sequencing read.
Burrows-Wheeler can align a sequencing read that matches two different exons.
Burrows-Wheeler uses a pre-compiled and compressed index of the reference genome.
Burrows-Wheeler can be parallelized.
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