2. A researcher attempts to repeat the experiment in which genetic engineering was used to transform cells in culture with the Xist gene. When the Xist gene was targeted to chromosome 21 in cells from a person with Down syndrome (trisomy 21), transcription of the copy of chromosome 21 containing Xist was shut down, much like the inactive X chromosome in cells from a normal female. After carrying out the experiment, the researcher measures the level of transcription averaged over a sample of genes in chromosome 21 and, as a control, measures the level of transcription of a sample of genes in chromosome 6. Shown here are several possible outcomes of such an experiment. Type of cell line Normal | 47,+21 1 47,+21 and Xist transformed 2.0 M 1.5 H K L 1.0 0.5 Chsm 6 Chsm 21 Chsm 6 Chsm 21 Chsm 6 Chsm 21 Chsm 6 Chsm 21 Chsm 6 Chsm 21 Biology: How Life Works, Third Edition O 2019 Macmillan Learning Which bar graph suggests that the experiment succeeded (that is, that the Xist gene became inserted into one of the copies of chromosome 21)? Briefly explain your answer. | Level of transcription

2. A researcher attempts to repeat the experiment in which genetic engineering was used to transform cells in culture with the Xist gene. When the Xist gene was targeted to chromosome 21 in cells from a person with Down syndrome (trisomy 21), transcription of the copy of chromosome 21 containing Xist was shut down, much like the inactive X chromosome in cells from a normal female. After carrying out the experiment, the researcher measures the level of transcription averaged over a sample of genes in chromosome 21 and, as a control, measures the level of transcription of a sample of genes in chromosome 6. Shown here are several possible outcomes of such an experiment. Type of cell line Normal | 47,+21 1 47,+21 and Xist transformed 2.0 M 1.5 H K L 1.0 0.5 Chsm 6 Chsm 21 Chsm 6 Chsm 21 Chsm 6 Chsm 21 Chsm 6 Chsm 21 Chsm 6 Chsm 21 Biology: How Life Works, Third Edition O 2019 Macmillan Learning Which bar graph suggests that the experiment succeeded (that is, that the Xist gene became inserted into one of the copies of chromosome 21)? Briefly explain your answer. | Level of transcription

Name: 2. A researcher attempts to repeat the experiment in which genetic en
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Description: 2. A researcher attempts to repeat the experiment in which genetic engineering was used to transform cellsin culture with the Xist gene. When the Xist gene was targeted to chromosome 21 in cells from a personwith Down syndrome (trisomy 21), transcri

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter16: Regulation Of Gene Expression

Section: Chapter Questions

Problem 10TYK

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1. Which of the following conditions will result to deactivation of a gene? a. histone methylation b. phosphorylation of a transcription factor c. binding of hormones to its nuclear receptor d. deactivation of transcription factor inhibitory protein 2. Direct complementarity of the microRNA to the target mRNA will lead to a. mRNA degradation b. inhibition of transcription c. chromatin remodeling d. inhibition of translation
1. Why aren't ssb proteins necessary in transcription?
1) Which of the following complexes contributes to the transcription of cyclin E? a) Cdk1/cyclin A b) Cdk2/cyclin B c) Cdk3/cyclin C d) Cdk4/cyclin D 2. DNA Polymerase δ (delta) has the following functions EXCEPT a) proof-read the new DNA strand. b) detach the dangling RNA primer. c) nip the RNA primer during termination. d) add DNA nucleotides to the lagging strand.
8 There is a promoter of a tumour suppressor gene in a mutant cancer cell line, and discover that instead of a nucleosome free region 5’ of the transcription start site there are nucleosomes bound. What would be the likely consequence on transcription of this gene? I am not sure about what is the outcome, normally it binds to the nucleosome free region 5' of the transcription start site to initiate transcription. Now, it binds with nucleosomes. What will happened? Is the DNA cannot transcripted successfully? Due to the transcription needs a naked DNA to process while in nucleosomes, DNA has been wrapped up. Thank you.
A binding .site for RNA polymerase is called a ___ . a. gene b. promoter c. codon d. protein
How do pseudogenes differ from genes? a. They are not transcribed. b. They contain longer open reading frames (ORFs). c. They do not have introns. d. They use a different genetic code. e. Their protein-coding sequence contains more than one startcodon.
Binding of ______ to _______ in DNA can increase the rate of transcription of specific genes. a. activators; repressors b. activators; enhancers c. repressors; operators d. both a and b
1)A. how do you read a sequence of DNA (template or non-template strand) to convert it an mRNA sequence and to a protein? B.How does chromatin remodeling regulate gene transcription? C. What are the major differences between gene expression in bacteria and eukaryotes D. How are non-coding regions involved in gene transcription? E. Explain how eukaryotic genes sometimes produce multiple protein products?
1. Transcription: a)State the role of RNA polymerase in gene transcription.b. Explain why the DNA is not used directly for protein translation (i.e., why is mRNA used instead?).c. Explain what occurs when a gene’s promoter region is open for RNA polymerase binding.d. Explain what occurs when a gene’s promoter regions is blocked from binding RNA polymerase.e. Explain how two cells, such as liver cells and skin cells, can become specialized in structure and function despite containing the same genome.
1.- Outline the general process of transcription (dna->rna) include a diagram a) include the basic enzymes involved and a description of their actions b) what is the product of transcription c)what will the cell do with the product d)where does the transcription take place in prokaryotic cell
2) what is negative control of genes ? Does it need active form or inactive form of the repressor ? 3) What is role of RNA polymerase, operator and the repressor in repressible operon and inducible operons ? 4) How is eukaryotic gene expression different than bacterial gene expression ? 5) How does histone acetylation influence transcription ?
1. Contrast positive versus negative regulation of gene expression. Describe the role of the repressor in an inducible system and in a repressible system.