2. A. B. C. The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. Human DNA is approximately 20% C. G=% A = % T= % In the bacteriophage DX174, the DNA is approximately 26% A. G= % T= % Sea urchin DNA is approximately 18% G. C = % C = % A = % T = %

2. A. B. C. The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. Human DNA is approximately 20% C. G=% A = % T= % In the bacteriophage DX174, the DNA is approximately 26% A. G= % T= % Sea urchin DNA is approximately 18% G. C = % C = % A = % T = %

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter12: Dna: The Carrier Of Genetic Information

Section: Chapter Questions

Problem 7TYU: The experiments in which Meselson and Stahl grew bacteria in heavy nitrogen conclusively...

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"Chargaff's rules" about the composition of bases in DNA dictates that A. the sum of purine residues must equal the sum of pyrimidine residues. B. the sum of A-T base pairs must equal the sum of G-C base pairs. C. the base composition of DNA is the same in all species. D. DNA specimens isolated from different tissues of the same species vary in base composition.
1. Below is the base sequence of protein for normal hemoglobin and the base sequence for the sickle cell hemoglobin:Normal: GGG CTT CTT TTTSickle: GGG CAT CTT TTTa. Transcribe and translate the normal and sickle cell DNA. b. Identify this as a point or frameshift mutation. Explain. c. If the base sequence read GGG CTT CTT AAA instead, would this result in sickle cell hemoglobin? Explain.
1. Which of the following are generated due to the twisting of dsDNA? a. Hydrophobic bonds b. Van der Waal forces c. Hydrogen bonds d. Grooves 2. Which of the following terms refers to the annealing of ssDNA to complementary RNA? a. Homologous combination b. Hybridization c. Denaturation d. Telomere lengthening 3. Which of the following completes this statement? The melting temperature of DNA is the temperature at which__________. a. DNA denatures b. DNA bonds are reinforced c. Solid DNA becomes liquid d. Liquid DNA coils
Watch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification Note: the video is entitled RNA but the method is identical for DNA quantification. 1. Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and 70% ethanol, in succession. What happens during these steps and why are they essential?2. Spectrophotometric detection of nucleic acids require readings at wavelengths of 260nm, and 280nm. What is the significance of these wavelengths?
1. a) If one DNA segment has the following base composition, 5'-CAGTTAGTCA-3', which of the following sequences is complementary? op 1: 3'-CAGTTAGTCA-5' op 2: 3'-TGACTAACTG-5' op 3: 5'-TGACTAACTG-3' op 4: 3'-TGACTAACTG-5' b) What is the nucleotide sequence of the DNA strand that is complementary to 5'-ATCGCAACTGTCACTA-3' op 1: 5'-TAGCGTTGACAGTGATA-3' op 2: 5'-TAGTGACAGTTGCGAT-3' op 3: 5'-ATCACTGTCAACGCTA-3' op 4: 5'-UAGUGACAGUUGCGAU-3'
Watch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification Note: the video is entitled RNA but the method is identical for DNA quantification. 1. At what ratio of A260/280 can we say that DNA is pure? What about RNA and protein?2. While spectrophotometric methods are effective at detecting DNA, a more sensitive but expensive technique called fluorometry is used in sensitive applications. What is the principle behind fluorometry and why is it better than spectrophotometry in detecting DNA?
Give 1 example of diseases arising from each of the following mutations and explain each. Point mutation Frameshift mutation Calculate for the DNA concentration. The absorbance at 260 nm is 0.7 at 1:30 dilution; the absorbance at 320 nm is 0.1. Is the DNA isolated of good quality? The absorbance at 260 nm is 0.7; the absorbance at 280 nm is 0.1; the absorbance at 320 is 0.01. If you do an absorbance reading after plasmid purification and get an A260/A280 of less than 1.8, how could you further purify the sample to get rid of the protein contamination? Is it always necessary to have completely pure DNA? What are some cases where it would or would not be?
1. Why do we need to check the isolated DNA for its quantity and quality? 2. The purity of DNA sample is below 1.8 A260/A280 so, where did the proteincontamination come from? Note: Cite the in-text citation and references
n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genes
An individuals set of unique _______ can be used in DNA profiling. a. DNA sequences b. short tandem repeats c. SNPs d. all of the above
When Griffith injected mice with a combination of live rough-strain and heat-killed smooth-strain pneumococci, he discovered that (a) the mice were unharmed (b) the dead mice contained living rough-strain bacteria (c) the dead mice contained living smooth-strain bacteria (d) DNA had been transferred from the smooth-strain bacteria to the mice (e) DNA had been transferred from the rough-strain bacteria to the smooth-strain bacteria
The experiments in which Meselson and Stahl grew bacteria in heavy nitrogen conclusively demonstrated that DNA (a) is a double helix (b) replicates semiconservatively (c) consists of repeating nucleotide subunits (d) has complementary base pairing (e) is always synthesized in the 5 3 direction