2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary stain, Counter stain, Decolorizing agent and Mordant)
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- Cite the purpose of each of the following reagents in a differential stainingprocedure.a. Primary stain:b. Mordant:Lab reportExPEriMEnt9 90 Experiment 9: lab reportc. Decolorizing agent:d. Counterstain:write the protocol for mann's methyl blue-eosin staining, including their reagents, materials, prinicple, procedures results, and cautionswrite the protocol for Hexamine silver staining, including their reagents, materials, prinicple, procedures results, and cautions
- 1. Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method. 3. What is the specific diagnostic value of this staining procedure?4. What is the specific diagnostic value of this staining procedure?5. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method.6. What is the specific diagnostic value of acid fast staining procedure?1.) What is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.
- Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid Schiff technique 2. Grocott's hexamine silver technique 3. Ziehl-Neelsen stain 4. Lactophenol blue stain 5. Mehtamine silver technique 6. Shikata's orcein method 7. Mann's methyl blue-eosin stain1. What is the significance of soaking the container in bleach? 2. What is the significance of keping the container closed except when pouring the medium; partially opening the container during pour-plating in preparing culture media?1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?