Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the following criteria: 1. Composition of gels 2. Dye used for staining
Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…
A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…
Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: components of a Romanowsky stain?
A: NOTE:- "As you have posted multiple questions under one, we will solve the first part for you, to…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b:…
A: According to bartleby expert guidelines, when multiple sub part questions are posted we are allowed…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: During acid fast staining first of all heat is used to fix the bacterial smear onto the slide.…
Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…
A: Gram staining may be a staining strategy utilized to distinguish and recognize bacterial living…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: Give the principle of acid fast staining and gram staining.
A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…
A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…
A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…
A: Staining is a technique usually employed to enhance the contrast of specimens in microscopy. Many…
Q: Molecules are tagged by antibodies in __________ microscopy. a. Confocal b. Differential…
A: Microscopy is defined as that technical field that makes use of microscopes in order to visualize…
Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…
A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…
Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…
A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.
Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…
A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…
Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…
A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…
A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…
Q: write the protocol for mann's methyl blue-eosin staining, including their reagents, materials,…
A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…
Q: What is safe view dye for? Compare it with Ethidium Bromide.
A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…
Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium
A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…
Q: name 5 things about method staining
A: INTRODUCTION Staining Staining is a method that is used in medical field and histology to see things…
Q: In order to have a better visualization of the shape acid the fast stain, you should do the…
A: Use of carbol fuchsin on the bacteria and heating it p, makes the cell appears red, presence of…
Q: Select all of the following that represent examples of positive stains. Check All That Apply…
A: Staining is the common laboratory procedure which can be specific and general according to the use.…
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…
A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…
Q: list the stains and decolorizing agent used in acid fast staining method.
A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…
Q: Why is it called as differential stain
A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: The staining is done to enhance the visualization of cell and certain cellular component under a…
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: Differentiate between agarose gel electrophoresis and SDS-PAGE based on composition of gels and dye…
A: Electrophoresis is the technique that is used to separate biomolecules that have a charge in them.…
Q: Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid…
A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not…
A: only acid fast appears red/pink. Only endospore appears green. Vegetative cell become colourless.
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary…
A: Differential Staining uses multiple chemical stains to view the microscopic organisms. It is because…
Q: Describe the procedure for performing a Gram stain. What is the procedure? How does each component…
A: * Gram staining is used to test that whether f bacteria is positive or negative and also be used to…
Q: k there are 2 staining reagents applied in differential s
A: Differential Staining- It is a staining process which uses more than one staining reagent. it is…
Q: Write down the materials, reagents, Principle, procedure and results for Ziehl-Neelsen stain
A: It is a differential staining technique which was first developed by Ziehl and later on modified by…
Q: differential staining procedures
A: Differential staining : Differential staining is one of the important staining method. In this…
Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…
A:
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain…
A: Cationic Dyes- These are the dyes which dissociates into cations in aqueous solutions. Anionic Dyes-…
Q: Name two conditions, which will affect the quality of your staining. B.What is a panoptic stain?…
A: Staining is a very important part in Histology, without which it would be impossible to study the…
Q: How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at…
A: Fluorescence spectroscopy uses a beam of light that excites the electrons that are present in the…
Q: ) Outline or discuss what happens to a gram negative and gram positive cell at EACH Individual step…
A: On the basis of lipid content and peptidoglycan layer in the cell wall, bacterias are classified…
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- Differentiate between agarose gel electrophoresis and SDS-PAGE based on composition of gels and dye used for staining.What is the application of Gel Electrophoresis in healthcare? Cite at least 2 specific examples.Explain the function of stacking gel, separating gel, and Coomassie blue stain in the electrophoresis experiment.
- what are two differences between agarose gel electrophoresis and SDS PAGE?list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet endospore staining methods. (omit the rinsing steps)Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.
- Why is polyacrylamide gel is used in electrophoresis?Outline the techniques of two-dimensional gel electrophoresisand tandem mass spectroscopy, and explain why they are used.What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.
- How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at your own words. Answer should be to the point(specific 4-5 lines).When run under nondenaturing conditions (without SDS or β-mercaptoethanol), nativepolyacrylamide gel electrophoresis (PAGE) allows proteins to maintain biological activity, so the GFP band should fluoresce when exposed to UV light. How do the staining patterns of gels run in this way differ from the staining patterns of gels run using SDS-PAGE?In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?