2. The reagent blank reading for a method to detect glucose gave the following fluorescence readings: 200, 215, 230, 222, and 212 RFU. Considering the calibration curve shown below, what is the limit of detection and limit of quantitation for this method? 8000 4000 2000 ya 51 993x 119 63 50 100 Glucose (pmol) RFU (EEm = 535/587)

2. The reagent blank reading for a method to detect glucose gave the following fluorescence readings: 200, 215, 230, 222, and 212 RFU. Considering the calibration curve shown below, what is the limit of detection and limit of quantitation for this method? 8000 4000 2000 ya 51 993x 119 63 50 100 Glucose (pmol) RFU (EEm = 535/587)

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.17QAP: Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC...

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