A spectrophotometric method for the quantitative analysis of Fe2+ in blood uses Zn2+ as an internal standard. A sample containing Fe2+ and Zn2+ with final concentration of 3.25 ppb and 0.75 ppb, respectively, that yields a signal of 0.423 for Fe2+ and 0.650 for Zn2+. Subsequently, a sample of blood is spiked with Zn2+ to give the same final concentration and yielded a signal of 0.832 for Zn2+ and 0.110 for the unknown concentration of Fe2+. Determine the concentration of Fe2+ in the sample of blood.

A spectrophotometric method for the quantitative analysis of Fe2+ in blood uses Zn2+ as an internal standard. A sample containing Fe2+ and Zn2+ with final concentration of 3.25 ppb and 0.75 ppb, respectively, that yields a signal of 0.423 for Fe2+ and 0.650 for Zn2+. Subsequently, a sample of blood is spiked with Zn2+ to give the same final concentration and yielded a signal of 0.832 for Zn2+ and 0.110 for the unknown concentration of Fe2+. Determine the concentration of Fe2+ in the sample of blood.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter24: Introduction To Spectrochemical Methods

Section: Chapter Questions

Problem 24.28QAP

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