2. Your supervisor instructs you to prepare chemically competent cells for heat shock transformation from old batches of competent cells available in the freezers. The competent cells would eventually be used for the expression of a prokaryotic enzyme using the PET vector system. (a) You found several different strains of Escherichia coli in the -80 C freezer. State three (3) E. coli strains you would use for your project.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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