2.1. For an enzyme (5µM), the following initial velocities have been reported depending on the substrate concentration: [Substrate], mM Vo 0.02 10.83 0.04 18.57 0.07 26.76 0.1 32.50 0.15 39.00 0.2 43.33 0.3 48.75 0.5 54.17 0.7 56.88 2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction 2.3. Determine Km and Vmax for this enzyme 2.4. Indicate where Vmax and Km can be recognized
2.1. For an enzyme (5µM), the following initial velocities have been reported depending on the substrate concentration: [Substrate], mM Vo 0.02 10.83 0.04 18.57 0.07 26.76 0.1 32.50 0.15 39.00 0.2 43.33 0.3 48.75 0.5 54.17 0.7 56.88 2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction 2.3. Determine Km and Vmax for this enzyme 2.4. Indicate where Vmax and Km can be recognized
Chapter13: Kinetic Methods
Section: Chapter Questions
Problem 4P
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Question
![2.1. For an enzyme (5µM), the following initial velocities have been reported depending
on the substrate concentration:
[Substrate], mM Vo
0.02
10.83
0.04
18.57
0.07
26.76
0.1
32.50
0.15
39.00
0.2
43.33
0.3
48.75
0.5
54.17
0.7
56.88
2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction
2.3. Determine Km and Vmax for this enzyme
2.4. Indicate where Vmax and Km can be recognized](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0aca0999-d9f2-47f4-928a-717e195168b4%2Fd50d5056-c225-4c87-9d4b-9c84b6775def%2Fjjuu39rc_processed.jpeg&w=3840&q=75)
Transcribed Image Text:2.1. For an enzyme (5µM), the following initial velocities have been reported depending
on the substrate concentration:
[Substrate], mM Vo
0.02
10.83
0.04
18.57
0.07
26.76
0.1
32.50
0.15
39.00
0.2
43.33
0.3
48.75
0.5
54.17
0.7
56.88
2.2. Sketch the Eadie-Hofstee plot for the enzyme reaction
2.3. Determine Km and Vmax for this enzyme
2.4. Indicate where Vmax and Km can be recognized
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