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23. Food additive is
A. Physical mutagen B. Chemical mutagen
C. Cosmetic agent
Step by step
Solved in 2 steps
- Mutation rates are typically low due toa. proofreading by DNA polymerase.b. DNA repair enzymes.c. silent mutations.d. None of these are correct.e. All of these are correct.1. The extraction of DNA is a common procedure in biotechnology research laboratories. Research the methods of extracting DNA in biotechnology facilities. Describe how the extraction methods used in this lesson compare to those of research laboratories.1. What is/are the purpose(s) of DNA extraction? 2. What is the function of each reagent used in the extraction of DNA from a banana? a. Saltwater b. Liquid dishwashing soap c. Cold isopropyl alcohol
- the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence after sequencing the proteins (b) shotgun sequence and assemble based on overlaps (c) identify mutations that affect glycolysis (d) obtain recombinant DNA clone maps before starting the sequencing (e) obtain comprehensive SNP maps before determining the order of DNA cloneGive 1 example of diseases arising from each of the following mutations and explain each. Point mutation Frameshift mutation Calculate for the DNA concentration. The absorbance at 260 nm is 0.7 at 1:30 dilution; the absorbance at 320 nm is 0.1. Is the DNA isolated of good quality? The absorbance at 260 nm is 0.7; the absorbance at 280 nm is 0.1; the absorbance at 320 is 0.01. If you do an absorbance reading after plasmid purification and get an A260/A280 of less than 1.8, how could you further purify the sample to get rid of the protein contamination? Is it always necessary to have completely pure DNA? What are some cases where it would or would not be?1. What happens in the denaturing step of PCR? A. What happens during the annealing step of PCR? B. What happens during the extension step of PCR? C. What temperatures is associated with each PCR step? D. Why is it necessary to have a primer on each side of the DNA segment to be amplified? (Hint: draw it out to see what happens with only one primer). E. How did the Taq DNA polymerase acquire its name? F. Why are there nucleotides (A, T, G, C) in the master mix? What are the other components of the master mix and what are their functions?
- Replicating dna polymerases have high fidelity A why is high fidelity more importsnt for dna polymerase than rna polymerase b list 2 mechanisms dna polymerase 3 used to reduce errors c despite high fidelity dna polymerase makes errors which type of sequence leads to strands slippage what what types of mutations occur ( transversion, insertion, deletion, transition ) when slippage occurs1A) Which of the following best explains Griffith’s transformation experiments? Two strains of S. pneumoniae were used for the experiment. Griffith injected a mouse with heat-inactivated S strain (pathogenic) and living R strain (non-pathogenic). The mouse died and living S strain was recovered from the dead mouse. From this, it was later concluded that that mutation occurred in the DNA of the R-strain cells thus transforming them into a pathogenic S strain. Two strains of S. pneumoniae were used for the experiment. Griffith injected a mouse with heat-inactivated S strain (pathogenic) and living R strain (non-pathogenic). The mouse died and living S strain was recovered from the dead mouse. From this observation, it was later concluded that external DNA from the inactivated S strain was taken up by the R-strain cells thus transforming them into a pathogenic S strain. Two strains of S. pneumoniae were used for the experiment. Griffith injected a mouse…The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.
- Practical applications of recombinant DNA technology include the: 1. Efficient production of useful proteins and development of a new type of vaccines 2. Creation of novel genotypes for the synthesis of economically important molecules. 3. Generation of DNA and RNA sequences for use in medical diagnosis. 4. all of the above WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger) 3. TALE (transcription activator light effector) 4. CRISPR COMMON DISEASE- COMMON VARIANT HYPOTHESIS STATES THAT 1. common disorders are likely influences by genetic variation that is also common in the population 2. if an SNP produced a deleterious mutation and changes amino acid sequence in 40% of individuals- it will produce disease phenotype in 40% individual 3. A SNP produce little change in gene expression- it will produce slight increase in risk/ manifestation of disease phenotype 4. all of the above WHAT IS TRUE REGARDING GENE ORGANIZATION IN THE NUCLEUS…Can you explain thie each of the statement given i dont really understand the dna recombinant is used as a molecular cloning and application for recombinant dnaWhy are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.