24-38. Use the method of half half reactions to balance the following equations: (po)010 (2) (HO)10 () (a) N₂H₁ (aq) + Cu(OH)₂ (s) → N₂(g) + Cu(s) (basic) TOUGHAL TAQI (b) H.AsO, (aq) + I₂(aq) → MAJAS H,AsO4 (aq) + I¯(aq) MOITAUON (acidic)
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- The speed with which the protein bovine serum albumin (BSA) moves through water under the influence of an electric field was monitored at several values of pH, and the data are listed below. What is the isoelectric point of the protein?pH 4.20 4.56 5.20 5.65 6.30 7.00Speed/(μm s-1) 0.50 0.18 -0.25 -0.65 -0.90 -1.25The velocity v with which a protein moves through water under the influence of an electric field varied with values of pH in the range 3.0 < pH < 5.0 according to the expression v/(μm s−1) = a + b(pH) + c(pH)2 with a = 0.80, b = −4.0 × 10−3, and c = −5.0 × 10−2. Identify the isoelectric point of the protein.Calculate the percentage ionization (i.e. [base]/[acid]x100) in these amino acid side chains at the pH values specified (show your work for one of these amino acid chains): - Aspartate (pKa=4.1) at pH 5.4 - Arginine (pKa=12.5) at pH 11.8 - Histidine (pKa=6.0) at pH 8.0 - Tyrosine (pKa=10.9) at pH 8.0
- -858kcal to mcalAcetic acid (CH3COOH, abbrev HA) is a very common weak acid with pKa = 4.75 (at 25oC and low ionic strength) A] Write down the dissociation equation of the weak acid HA in aqueous solution, derive the respective formulae for the dissociation constant Ka and pKa. B] Calculate pH of the diluted solution of acetic acid with concentration, c = 0.05 mol/dm3 C] What is the approximate pH of the aqueous acetate buffer solution composed of dissolved mixture of acetic acid (HA) and sodium acetate (NaA) in the same respective concentrations? What is the working pH range of acetate buffers? D] How can you prepare exactly 1 dm3 of acetate buffer solution with required pH 5.0 and the total concentration [HA + NaA], c = 0.01 mol/dm3 if the stock solution of acetic acid (HA) with concentration, cHA = 0.1 mol/dm3, anhydrous sodium acetate (NaA, MT = 82.03 g/mol) and purified water are available? E] Acetate buffer solution may be also prepared by the partial neutralization of HA with NaOH: 20 cm3 of…You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
- Determine the buffer capacity of Alanine and Histidine at pH 2.0 and 6.0 respectively and discuss the results.The Racah parameter B for [Co(CN)6]3−=460cm−1, and [Co(NH3)6]3+= 615cm−1. Explain the difference in nephelauxetic effect. Thank you!Draw a sketch of the Langmuir isotherm at a pressure of 0.001kPa-1.
- Extraction of DNA involves the use of a 1 M phosphate buffer (1-liter volume) having the hydrogen ion concentration of 6.165950019 x 10-9 M. Three pKa of phosphoric acid are available Concentrated phosphoric acid (85% w/w; Sp. Gr. = 1.70; MW = 98 g/mol) Sodium dihydrogen phosphate monohydrate (MW = 138g/mol) Sodium biphosphate heptahydrate (MW = 268g/mol) Tribasic sodium phosphate monohydrate (MW = 182 g/mol) Sodium hydroxide pellets (40 grams/mol) Question: What is the pH of the solution? (answer in two decimal places) How much of the weak acid component should be used? (Note for answer: Round to the nearest hundredth and include the unit such as "grams or milliliters"; Ex. 100.00 grams or 100.00g for weight & 100.00 milliliters or 100.00 mL for volume) Question: What is the suitable buffer component to be used? (Note for the answer: Separate the two components with “and”; Ex: Ammonia and Ammonium chloride) Question: How much of the salt component should be used?…asdbas14Calculate the binding affi nity of a ligand for its receptor from the following data: