A) Make 500 ml of a 50 X solution of HEPES buffer. B) Use this (50X HEPES Buffer) to make 1 liter of 0.5X HEPES Buffer solution. Write out how you would do this and include the concentration of each ingredient in the 0.5X Buffer. GIVEN: RECIPE FOR 1X HEPES Buffer ( 1 Liter) NaCl: 115 mM (FW: 58.44). CaCl2:1.2 mM (FW: 110.98) MgCl₂ :1.2 mM (FW: 95.21) K₂HPO4 :2.4 mM (FW: 174.18) HEPES: 20 mM (FW: 238.30) Adjust pH to 7.4.

Appl Of Ms Excel In Analytical Chemistry
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Chapter8: Polyfunctional Acids And Bases
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Please give me correct solution .
A) Make 500 ml of a 50 X solution of HEPES
buffer.
B) Use this (50X HEPES Buffer) to make 1
liter of 0.5X HEPES Buffer solution. Write
out how you would do this and include the
concentration of each ingredient in the 0.5X
Buffer.
GIVEN:
RECIPE FOR 1X HEPES Buffer ( 1 Liter)
NaCl: 115 mM (FW: 58.44).
CaCl2:1.2 mM (FW: 110.98)
MgCl₂ :1.2 mM (FW: 95.21)
K₂HPO4 :2.4 mM (FW: 174.18)
HEPES: 20 mM (FW: 238.30)
Adjust pH to 7.4.
Transcribed Image Text:A) Make 500 ml of a 50 X solution of HEPES buffer. B) Use this (50X HEPES Buffer) to make 1 liter of 0.5X HEPES Buffer solution. Write out how you would do this and include the concentration of each ingredient in the 0.5X Buffer. GIVEN: RECIPE FOR 1X HEPES Buffer ( 1 Liter) NaCl: 115 mM (FW: 58.44). CaCl2:1.2 mM (FW: 110.98) MgCl₂ :1.2 mM (FW: 95.21) K₂HPO4 :2.4 mM (FW: 174.18) HEPES: 20 mM (FW: 238.30) Adjust pH to 7.4.
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