4. Questions about Figure 3 (Figure 5 in the paper) below. This was an examination of different pairs of B and y subunits and their ability to localize to the plasma membrane. A В C YN-B,YC-Y1 YN-B YC-Y7 YN-B,YC-Y2 YN-B2YC-Y7 YN-B2YC-Y2 YN-B,YC-Y 2 YN-BYC-Y7 YN-BYC-Y2 YN-BSYC-y LL I
a. Panels G, H, and I show pattters wher fluorescence is detected in the cytosol or internal cellular membranes. Do these results support the hypothesis that the identity of the beta subunit also plays a role in subcellular localization?
b.If co-expression of a given alpha subunit caused the pairs in panels G, H, and I to migrate to the plasma membrane, what would that tell you about the process of plasma membrane localization with heterotrimeric G-proteins containing the beta-5 subunit?
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