Proteins that are retained in the ER have a KDEL sequence at their C-terminus. To test the idea that KDEL is both necessary and sufficient for retention in the ER, two experiments were performed. First, it was deleted from protein disulfide isomerase (PDI), and this form of PDI got secreted. Second, it was attached to a peptide growth hormone, which is normally secreted from the cell. This modified peptide still got secreted. Which interpretation does this support? The data do not allow for an interpretation, additional experiments are needed. KDEL is sufficient but not necessary for ER retention. KDEL is both necessary and sufficient for ER retention. KDEL is necessary but not sufficient for ER retention.

Protein disulfide isomerase,, is an enzyme in the endoplasmic reticulum (ER) in eukaryotes that…

Proteins that are retained in the ER have a KDEL sequence at their C-terminus. To test the idea that KDEL is both necessary and sufficient for retention in the ER, two experiments were performed. First, it was deleted from protein disulfide isomerase (PDI), and this form of PDI got secreted. Second, it was attached to a peptide growth hormone, which is

Proteins that are retained in the ER have a KDEL sequence at their C-terminus. To test the idea that KDEL is both necessary and sufficient for retention in the ER, two experiments were performed. First, it was deleted from protein disulfide isomerase (PDI), and this form of PDI got secreted. Second, it was attached to a peptide growth hormone, which is

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 13QP: Suppose that in the formation of phenylalanine hydroxylase mRNA, the exons of the pre-mRNA fail to...

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a. Panels G, H, and I show pattters wher fluorescence is detected in the cytosol or internal cellular membranes. Do these results support the hypothesis that the identity of the beta subunit also plays a role in subcellular localization? b.If co-expression of a given alpha subunit caused the pairs in panels G, H, and I to migrate to the plasma membrane, what would that tell you about the process of plasma membrane localization with heterotrimeric G-proteins containing the beta-5 subunit? (This is all the information that is provided!)
When the amino acid levels in eukaryotic cells are low, general protein synthesis is reduced. Gcn4 translation, however, is increased. A. What would happen under high and low amino acid conditions if only one of the upstream ORFs were deleted from Gcn4? B. What would happen under high and low amino acid conditions if all of the upstream ORFs were deleted from Gcn4?
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When the amino acid levels in eukaryotic cells are low, general protein synthesis is reduced.Gcn4 translation however is increased.what would happen under high and lowamino acid conditions if only one of the upstream ORFs were deleted from Gcn4?
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		The data do not allow for an interpretation, additional experiments are needed.
		KDEL is sufficient but not necessary for ER retention.
		KDEL is both necessary and sufficient for ER retention.
		KDEL is necessary but not sufficient for ER retention.