4. TLC is still useful in the lab but is being replaced by automated chromatographic methods. For example, TLC, FPLC, and HPLC are now automated processes, and computers can control various parameters, such as flow rate, blends of solvent mixtures, etc. Can you think of how the ability to manipulate the parameters of these automated chromatographic methods (sample size, column plate length, flow rate, solvent gradients, etc.) might accentuate or improve an investigator's ability to separate amino acids or other biomolecules?
4. TLC is still useful in the lab but is being replaced by automated chromatographic methods. For example, TLC, FPLC, and HPLC are now automated processes, and computers can control various parameters, such as flow rate, blends of solvent mixtures, etc. Can you think of how the ability to manipulate the parameters of these automated chromatographic methods (sample size, column plate length, flow rate, solvent gradients, etc.) might accentuate or improve an investigator's ability to separate amino acids or other biomolecules?
Chapter8: Forms Of Drugs And How They Act
Section: Chapter Questions
Problem 30RQ
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Automated chromatography is a process in which the separation of molecules is carried out automatically by a computer-controlled system.
The system can be programmed to adjust various parameters such as flow rate, solvent mixtures, column temperature, and detection parameters to optimize the separation of specific compounds or biomolecules.
There are several types of automated chromatographic methods, including:
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FPLC (Fast Protein Liquid Chromatography)
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HPLC (High-Performance Liquid Chromatography)
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GC (Gas Chromatography)
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TLC (Thin Layer Chromatography)
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