Answer this question O Macmillan LearningThe measurement of alanine aminotransferase activity (reaction rate) usually includes an excess of pure lactate dehydrogenaseand NADH in the reaction system. The rate of alanine disappearance is equal to the rate of NADH disappearancemeasuredspectrophotometrically.How does this assay work?NADH is the substrate for alanine synthesis. Alanine aminotransferase metabolizes alanine at the same rate asalanine synthesis.O Alanine and NADH are substrates for alanine aminotransferase. The reaction equilibrium favors alanine and lactatedehydrogenase is added to remove the product.O Alanine aminotransferase and lactate dehydrogenase metabolize alanine and NADH at the same rate, respectively.Alanine aminotransferase requires the cofactor NADH to metabolize alanine.Alanine aminotransferase catalyzes the rate-limiting reaction in a coupled-reaction assay. Lactate dehydrogenaserapidly metabolizes the pyruvate product, using NADH, as quickly as it forms.

If a particular reaction brings about a measurable change (like the generation or consumption of a…

Answered: The measurement of alanine…

Biochemistry

6th Edition

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Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter19: The Tricarboxylic Acid Cycle

Section: Chapter Questions

Problem 16P

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The measurement of alanine aminotransferase activity (reaction rate) usually includes an excess of pure lactate dehydrogenase and NADH in the reaction system. The rate of alanine disappearance is equal to the rate of NADH disappearance measured spectrophotometrically. How does this assay work?