4. Tris(hydroxymethyl)aminomethane [(HOCH2)3CNH2–Tris, or THAM] is a weak base frequently used to prepare buffers in biochemistry. Its Kb is 1.2 x 10-6 and pKp is 5.92. The corresponding pKa is 8.08, which is near the pH of the physiological buffers, and so it exhibits good buffering capacity at physiological pH. What weight of THAM must be taken with 100 mL of 0.50 M HCl to prepare 1 L of a pH 7.40 buffer?

4. Tris(hydroxymethyl)aminomethane [(HOCH2)3CNH2–Tris, or THAM] is a weak base frequently used to prepare buffers in biochemistry. Its Kb is 1.2 x 10-6 and pKp is 5.92. The corresponding pKa is 8.08, which is near the pH of the physiological buffers, and so it exhibits good buffering capacity at physiological pH. What weight of THAM must be taken with 100 mL of 0.50 M HCl to prepare 1 L of a pH 7.40 buffer?

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter8: Polyfunctional Acids And Bases

Section: Chapter Questions

Problem 3P

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Tris(hydroxymethyl)aminomethane [(HOCH2)3CNH2—Tris, or THAM] is a weak base frequently used to prepare buffers in biochemistry. Its Kb is 1.2 × 10−6 and pKb is 5.92. The corresponding pKa is 8.08, which is near the pH of the physiological buffers, and so it exhibits good buffering capacity at physiological pH. What weight of THAM must be taken with 100 mL of 0.50 M HCl to prepare 1 L of a pH 7.40 buffer?
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