4. Which media discussed in this exercise would be best for checking a water sample for potential E. coli contamination from sewage? Explain your answer.

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter26: Prokaryotes: Bacteria And Archaea
Section: Chapter Questions
Problem 4TYK: At the health center, a fecal sample was taken from a feverish student. Organisms with corkscrewlike...
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help on question 4

1. In addition to nutrients Lowenstein-Jensen (L-J) medium (used to culture
Mycobacterium species including M. tuberculosis) contains malachite green (a cytotoxin)
and antibiotics (penicillin and nalidixic acid) which inhibit both Gram negative and
positive bacteria. This would be an example of what kind of media?
2. What is it about Mycobacterium (compared to other bacteria) that allows for L-J media
to even work (e.g, to be useful for this purpose)?
3. What is it about blood agar that makes it useful for discriminating pathogenic strains of
Streptococci from non-pathogenic (normal flora) strains? Be specific.
4. Which media discussed in this exercise would be best for checking a water sample for
potential E. coli contamination from sewage? Explain your answer.
5. Which media discussed in this exercise would be best for checking a food sample for the
presence of Staphylococcus aureus, a common cause of food poisoning? Explain your
answer.
Transcribed Image Text:1. In addition to nutrients Lowenstein-Jensen (L-J) medium (used to culture Mycobacterium species including M. tuberculosis) contains malachite green (a cytotoxin) and antibiotics (penicillin and nalidixic acid) which inhibit both Gram negative and positive bacteria. This would be an example of what kind of media? 2. What is it about Mycobacterium (compared to other bacteria) that allows for L-J media to even work (e.g, to be useful for this purpose)? 3. What is it about blood agar that makes it useful for discriminating pathogenic strains of Streptococci from non-pathogenic (normal flora) strains? Be specific. 4. Which media discussed in this exercise would be best for checking a water sample for potential E. coli contamination from sewage? Explain your answer. 5. Which media discussed in this exercise would be best for checking a food sample for the presence of Staphylococcus aureus, a common cause of food poisoning? Explain your answer.
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