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1. TRUE or FALSE. An autoclave can be used to sterilize growth media?
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- which of the following is best to sterilize heat-liable (sensitive) solutions? a, pasteurization b. autoclave c. dry heat d. filration e. none of the above1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C. Autoclave d. Sun lightAgar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal for cultivation of microbes? c. How and why does the agar concentration in semisolid media differ from conventional solid media?
- 1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilledAgar is a solidifying agent used in media preparation. What makes it ideal for cultivation of microbes?1. Give the method of sterilization of these laboratory apparatuses how they are sterilized; inoculating loop, glasswares. culture media, skin, and milk. Please cite where you got the information. Thank you
- 15. Agar is used for solidifying culture media because: it does not affect the growth of bacteria it does not add to the nutritive properties of the medium the melting and solidifying points of agar solution are not the same all of the above1. Describe and give the principle of each equipment used for sterilization in microbiology. (refer to equipment drawn only)1. Give examples of specific applications for different types of solid tubed media 2. Give 5 examples of microorganisms that may be grown in Nutrient Broth. 3. Discuss the implication/s if the spore strip was missed out during the preparation of the sample for autoclave and possible remedy for this scenario.
- 1. In an autoclave, how are temperatures above boiling achieved?(short answer) 2.. Can culture media be sterilized in the hot air sterilizer? Why or why not? (short answer) 3.What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame? (short answer)Can culture media be sterilized in the hot air sterilizer? Why or why not?3. Principles and methods of sterilization