3A.) A throat and nasal sample are taken from a patient to determine the type of bacterial infection they are suffering from. Would a gram stain help clearly identify the bacteria responsible for causing the infection? Why or why not? What else would be found there? (Think critically) 3B.) What other types of test could you do to determine what is causing the infection? (You may need to do a little outside research)

3A.) A throat and nasal sample are taken from a patient to determine the type of bacterial infection they are suffering from. Would a gram stain help clearly identify the bacteria responsible for causing the infection? Why or why not? What else would be found there? (Think critically) 3B.) What other types of test could you do to determine what is causing the infection? (You may need to do a little outside research)

Essentials of Pharmacology for Health Professions

7th Edition

ISBN:9781305441620

Author:WOODROW

Publisher:WOODROW

ChapterCRE2: Comprehensive Review Exam For Part 2

Section: Chapter Questions

Problem 5CEP2

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3A.) A throat and nasal sample are taken from a patient to determine the type of bacterial infection they are suffering from. Would a gram stain help clearly identify the bacteria responsible for causing the infection?
1. What kinds of media would be used to culture and Identify this microbe?2. What are some other potential microbes that could have this infection?
Which microscopy technique is BEST at determining the presence of physical microbial interaction(s)? a Light microscopy. b Scanning or transmission electron microscopy. c Fluorescent microscopy. d Phase contrast microscopy.
1.) What is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.
(a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside from bacteria, can gram staining be used with microbes such as fungi? Explain your answer.
a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.
A 82-year-old female patient presents with a non-productive cough that often ends in wheezing , and difficulty breathing with painful inhalations. A. What specimen might you collect? B. What tests should be run on the sample and the patient? C. What might be the cause of this respiratory infection? D. Proposed treatment?
1. What is the significance of soaking the container in bleach? 2. What is the significance of keping the container closed except when pouring the medium; partially opening the container during pour-plating in preparing culture media?
1. What is the most common sterilization technique used in laboratories? 2. List at least 5 procedures of the aseptic technique and describe its uses. 3. Why is Gram stain one of the most important and widely used stains in bacteriology? 4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.
1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?
From figure . What type of bacterial infection could bediagnosed using the stain shown in the figure? What does this resultindicate?
5. You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.