5. State whether the following statements are True or False, If False, explain why 5.1 Oxaloacetate is formed by the oxidation of L-malate 5.2 An overstimulation of the citric acid cycle will cause an overproduction of oxygen 5.3 The citric acid cycle occurs in the mitochondrial matrix of prokaryotes and eukaryotic 5.4 NADH and ADP are high energy electron carriers which can be utilised in the electron transport chain
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- Ethanol as a Source of Metabolic Energy (Integrates with Chapters 19 and 20.) Acetate produced in ethanol metabolism can be transformed into acetyl-COA by the acetyl thiokinase reaction: Acetate+ATP+CoASHacetyleCoA+AMP+PPiAcetyle-CoA then can enter the citric acid cycle and undergo oxidation to 2 CO2by this route, assuming oxidative phosphorylation is part of the process? (Assume all reactions prior to acetyl-CoA entering the citric acid cycle occur outside the mitochondrion). Per carbon atom, which is a better metabolic fuel, ethanol or glucose? That is, how many ATP equivalents per carbon atom are generated by combustion of glucose versus ethanol to CO2?Complete oxidation of a 16-carbon fatty acid can yield 129 molecules of ATP Study Figure 19.2 and determine how many ATP molecules would be generated if a 16-carbon fatly acid were metabolized solely by the TCA cycle, in the form of S acetyl-CoA molecules.The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)
- ndicate whether each of the following statements is true or false. If false, correct the statement or provide a briefexplanation for why it is false.g. The high potential electrons of NADH enter the respiratory chain at NADH-Q reductase.h. Ubiquinol (QH2) is also the entry point for electrons from NADH of flavoproteins.i. Cytochrome reductase catalyzes the transfer of electrons from cytochrome c to O2.Consider a 24:1 △cis-9 fatty acid in the mitochondrion. For each fatty acid given, determine the following. 1. Gross ATP from b-oxidation cycles 2. Gross ATP from acetyl CoA produced 3. Gross ATP from conversion of propionyl CoA (if applicable) 4. Total number of ATP deducted 5. Total net ATPOxidation of NADH and FADH2 in the electron transport system are similar in that: (READ THESE ANSWER CHOICES CAREFULLY!) a Both give their electrons to Complex I. b Both give their electrons directly to Ubiquinone (CoQ). c Both require Ubiquinone, Complex III, Cytochrome C, and Complex IV, ultimately resulting in reduction of O2 d Both result in pumping of H+ from the intermembrane space to the mitochondrial matrix.
- Consider a brain cell with non-functional Complex II of the electron transport chain (Assume that the deficiency is isolated and will not influence the function of other respiration components) In these cells/tissues, determine the following from the catabolism of the 2.5 moles of the disaccharide lactose (will be hydrolyzed first to yield glucose and galactose). Net ATP from glycolysis ATP from oxidative decarboxylation (if applicable) ATP formed from Krebs cycle (if applicable) Total net ATPGiven the overall equation for respiration (C6H12O6 + 6O2 6CO2 + 6H2O), in which step(s) in the respiratory pathway is/are each of the reactants used up and each of the products made? Photosynthetic reactions require NADPH while NADH is involved in aerobic respiration. These two molecules regulate both the biosynthetic and the catabolic activity of the cell. Explain how they do this. Note that NAD+ kinase phosphorylates NAD+ to produce NADPH+.Although molecular oxygen (O2) does not participate directly in any of the reactions of the citric acid cycle, the cycle operates only when O2 is present. Explain this observation.
- Biochemists consider the citric acid cycle to be the cen-tral reaction sequence in metabolism. One of the key steps isan oxidation catalyzed by the enzyme isocitrate dehydrogenaseand the oxidizing agent NAD. Under certain conditions, thereaction in yeast obeys 11th-order kinetics Rate=k[enzyme][isocitrate]⁴[AMP]²[NAD⁺]m[Mg²⁺]² What is the order with respect to NAD⁺?Calculate the ATP yield for the complete oxidation of the ketone body 3-hydroxybutryate to 4 CO2 in the mitochondrial matrix. Use P:O ratios of 2.5 for NADH and 1.5 for QH2. Draw the chemical reaction and the movement of electrons (including the enolate intermediate) necessary for the spontaneous decarboxylation of acetoacetate to acetone and CO2. (Hint: review the mechanism of step 4 in glycolysis and step 3 in the citric acid cycle). Why is acetoacetate undergoing spontaneous decarboxylation while 3-hydroxybutyrate does not?Normally, the flow of electrons to O2 is tightlylinked to the production of ATP via the electrochemicalgradient. If ATP synthase is inhibited, for example, elec-trons do not flow down the electron-transport chain andrespiration ceases. Since the 1940s, several substances—such as 2,4-dinitrophenol—have been known to uncou-ple electron flow from ATP synthesis. Dinitrophenol wasonce prescribed as a diet drug to aid in weight loss. Howwould an uncoupler of oxidative phosphorylation pro-mote weight loss? Why do you suppose dinitrophenol isno longer prescribed?