6. An 80-year-old man patient was admitted to the hospital with a fever of 102 F. A sputum culture revealed many gram-negative rods on MacConkey agar and blood agar. The patient was diagnosed with pneumonia. The following biochemical results were obtained from the culture: H;S = Neg %3D Lactose = + Urease = + Citrate = + Indole = + VP = + Motility = Neg Resistance to ampicillin and carbenicillin %3D Name organism:
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- 1. Why do you think the 5% sheep blood agar was selected for the culture of mouth bacteria?1. What is the most common sterilization technique used in laboratories? 2. List at least 5 procedures of the aseptic technique and describe its uses. 3. Why is Gram stain one of the most important and widely used stains in bacteriology? 4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.1. The selective ingredient in mannitol salt agar is ______________, which selects for the growth of Staphylococcus organisms.
- 1. List multiple reasons why preventing the growth of certain microbes is necessary/valuable 2. For bacterial growth, what can we do to compose a continuous culture where we would get continuous supply of the product? 3. Antibiotics - How did we compose major issues for treatment through resistance built up from the antibiotics?1. Why do you disinfect your work area at the beginning of lab and then again at the end of lab? 2. If you have used proper aseptic technique to inoculate a new test tube of nutrient broth from apure culture of Bacillussubtilis, how many types of bacteria do you expect to grow in the new broth?And why?2. A mannitol-salt agar plate was inoculated with these bacteria and is shown below. A. What type of organisms grow on this medium? B. Based on the reaction below, what can you say about the organism derived from the patient's abscess?
- 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…6)Which of the following statements is a reason why Acid-Fast Bacteria resist the Gram stain a) Because their cell wall architecture varies from both gram-negative and gram-positive bacteria. b) Because they lack the peptidoglycan layer. c) Because they have a thick peptidoglycan layer. d) Because they have a thick layer of fatty acids.1. What is the influence of pH and age of the cultures on Gram reaction? Explain this comprehensively and please, do not just copy form somewhere.
- 3. which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation, catalase test, oxidase test, PCR test, urea hydrolysis test, aglutination test, northern and southern test.) can be used on the disease causing organism below that can help draw a flow chart or ditochomy key? And will the test results in positive or negative to these these. AcneRabiesGonorrhea Variant Creutzfeldt-Jakob diseaseLyme disease Shigellosis Gas gangrenePinworm infestation Pityriasis versicolor Tuberculosis1. Illustrate in a diagram the identification scheme from gram-positive cocci.2. Discuss the principle involved in the biochemical tests in identifying species of staphylococci.3. Illustrate in a diagram the identification scheme for Streptococci.4. Discuss the principle involved in the biochemical tests in identifying species of Streptococci.1. The technique used in bacteria for endospore staining can also be used to observe fungal spores. Select one: True False 2. Which of the following statements is/are TRUE? a. Agar does not provide nutrients to microorganisms. b. Peptone, pork extracts, and yeast extracts provide nutrients to microbes. c. Tap water must be used for preparing culture media. d. A & B are correct. e. B & C are correct Clear my choice 3. Motility of microorganisms is BEST observed with a a. Flagellar stain b. Hanging drop preparation c. Solid medium d. Negative stain e. Streak plate Clear my choice