97 Which of the following is examples of a transposable element found in bacteria? (multiple choice questions) A. IS903 b. Tn5 c. IS1 D. Xis 98 What is the key component of the catalytic site of the spliceosome? (multiple choice questions) A. DNA b. ribosomes c. protein D. RNA 99 Which antibiotic can binds to RNA polymerase and blocks an early step in RNA synthesis? A. Ampicillin b. Chloramphenicol c. Rimantidine D. None of the above

97 Which of the following is examples of a transposable element found in bacteria? (multiple choice questions) A. IS903 b. Tn5 c. IS1 D. Xis 98 What is the key component of the catalytic site of the spliceosome? (multiple choice questions) A. DNA b. ribosomes c. protein D. RNA 99 Which antibiotic can binds to RNA polymerase and blocks an early step in RNA synthesis? A. Ampicillin b. Chloramphenicol c. Rimantidine D. None of the above

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter16: Gene Expression

Section: Chapter Questions

Problem 9RQ: The a/a operon is an inducible operon that controls the production of the sugar arabinose. When...

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73Dicer catalyzes Sequence-dependent single-stranded RNA cleavage. Yesorno 74Amino acid-coding regions within eukaryotic genes may be interrupted by ___________ regions. A.noncodingb.long terminal repeatc.split geneD.enhancer 75Which of the following cleaves the N-terminal methionine from polypeptides?A.Methionine isomeraseb.Methionine aminopeptidasec.Methionine carboxylaseD.Methionine transferase
3. Jacques Monod, one of the geneticists who won the NobelPrize in medicine for deciphering the regulatory control of thelac operon, famously stated:“All that is true for E. coli, is true for the elephant.” • In 2-3 sentences, provide a rationale for why Monod’sstatement accurately reflects fundamental shared featuresof gene regulatory control in common between prokaryotesand eukaryotes. • In an additional 2-3 sentences, provide a critical analysisthat describes in detail AT LEAST TWO of the keydifferences between prokaryotes and eukaryotes in howthey regulate their genes.
Q11. Ribosomes are “ribonucleoprotein particles” in that they are composed mostly of rRNA with some associated ribosomal proteins. How are the genes coding for ribosomal RNAs the same as the genes coding for ribosomal proteins? They both have a transcriptional start site. They both have similar open reading frames to facilitate binding. They both have a transcriptional terminator. They both suffer frameshift mutations with the insertion of 2 nucleotides. A. 1, 2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct
1) The -10 and -35 regions of prokaryotic promoters are called consensus sequences because _________? a. they are identical in all bacterial species b. they are similar in all bacterial species c. they exist in all organisms d. they have the same function in all organisms 2) During translation elongation, in which site it the new peptide bond formed? a. A site b. E site c. P site d. small ribosomal subunit
Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which alsoserved as a model system for the study of bacteriophage morphogenesis, DNA replication, andgene regulation.a) With the aid of a diagram, generally narrate how a foreign gene can be inserted into alambda insertion vector and subsequently infect an Escherichia coli cell.b) You are cloning a 7.5 kb gene into a lambda gt10 vector, utilizing a restriction site whichspecifically present in the vector. State the restriction site that you can use for this purposeand suggest a screening procedure to indicate successful integration of the gene into thehost genome.
Refer to the DNA sequence provided: 3’ -TACTGAAGCGGCAGCCCCGCATGAGTAGACCTTACT-5’ a. What is the mRNA transcript of the anticoding strand of the DNA model? b. What is the amino acid sequence of the polypeptide chain that will be translated from the mRNA in (a)?
How do eukaryotic and prokaryotic RNA polymerases compare? a. Since eukaryotic RNA polymerases are more complex, no homology has been found between the actual protein sequences in the catalytic subunits. b. Since eukaryotic transcription is less complex than in prokaryotes, monomeric, single polypeptide RNA polymerases are used. c.Despite their added complexity, eukaryotic and prokaryotic RNA polymerases are generally homologous and share some conserved sequences d. Eukaryotic and prokaryotic RNA polymerases are virtually identical.
82Transcription termination takes place downstream from the _______________. A.poly(A) siteb.promoter regionc.5'-m7 G capsD.enhancer sequences 83The core promoter element of RNA polymerase II extends from about 40 bp upstream of the transcription initiation site to about _______ bp downstream from this site. A.100b.1000c.40D.200 84Archaeal and eukaryotic ribosome structure appear to be different from the bacterial ribosome structure. Yesorno
88Sequential binding of RNA polymerase II-TFIIF complex, TFIIE, and TFIIH completes ___________________ formation. A.pre-initiation complexb.TF recognition elementc.pre-elongation complexD.TATA binding complex 89Which of the following is the GDP-GTP exchange protein?A.EF-Tub.none of the abovec.EF-TsD.EF-G 90RNA polymerase II has 14 subunits. Yesorno
Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect would a point mutation at any one of the bolded and underlined nucleotides disrupt termination of transcription? Group of answer choices Mutation in one of these nucleotides would disrupt base pairing, preventing the formation of the hairpin and disrupting termination. Mutation in one of these nucleotides would have no affect on base pairing, so the termination hairpin is formed and termination proceeds. Mutation in one of these nucleotides would not disrupt base pairing, but would prevent the formation of the hairpin and disrupt termination. Mutation in one of these nucleotides would disrupt base pairing, but not affect the formation of the hairpin and termination proceeds.
70Pre-initiation begins with either TFIIF or TATA binding protein binding to the core promoter. Yesorno 71Exons tend to be non-conserved during evolution, whereas introns usually are conserved. Yesorno 72Transfer RNA molecules fold into L-shaped three-dimensional structures. Yesorno
. You are studying proteins having roles in translation inthe mouse. By BLAST analysis of the predicted proteinsof the mouse genome, you identify a set of mouse genesthat encode proteins with sequences similar to those ofknown eukaryotic translation-initiation factors. You areinterested in determining the phenotypes associatedwith loss-of-function mutations of these genes.a. Would you use forward- or reverse-genetics approaches to identify these mutations?b. Briefly outline two different approaches that youmight use to look for loss-of-function phenotypes in oneof these genes.