. You are studying proteins having roles in translation inthe mouse. By BLAST analysis of the predicted proteinsof the mouse genome, you identify a set of mouse genesthat encode proteins with sequences similar to those ofknown eukaryotic translation-initiation factors. You areinterested in determining the phenotypes associatedwith loss-of-function mutations of these genes.a. Would you use forward- or reverse-genetics approaches to identify these mutations?b. Briefly outline two different approaches that youmight use to look for loss-of-function phenotypes in oneof these genes.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
. You are studying proteins having roles in translation in
the mouse. By BLAST analysis of the predicted proteins
of the mouse genome, you identify a set of mouse genes
that encode proteins with sequences similar to those of
known eukaryotic translation-initiation factors. You are
interested in determining the
with loss-of-function mutations of these genes.
a. Would you use forward- or reverse-genetics approaches to identify these mutations?
b. Briefly outline two different approaches that you
might use to look for loss-of-function phenotypes in one
of these genes.
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