A 2.00 g serum sample is analyzed for protein by the modified Kjeldahl method. The sample is digested and the ammonia is distilled into 50.0 mL of 1.00 M boric acid (FW=61.84 g/mol). A volume of 15.0 mL of standard HCI is required for the titration of the ammonium borate to the methyl orange endpoint. The HCI is standardized by treating 0.330 g of pure sodium carbonate (FW=105.99 g/mol) in the same manner. If 33.3 mL of the acid is required in the standardization process, what is the % protein in the serum? The protein is known to contain 16.0%N. (nitrogen AW=14.01 g/mo

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter17: Complexation And Precipitation Reactions And Titrations
Section: Chapter Questions
Problem 17.35QAP
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A 2.00 g serum sample is analyzed for protein by the modified Kjeldahl method. The sample is digested and the ammonia is distilled into 50.0 mL of 1.00 M boric acid (FW=61.84 g/mol). A volume of 15.0 mL of standard HCI is required for the titration of the ammonium borate to the methyl orange endpoint. The HCI is standardized by treating 0.330 g of pure sodium carbonate (FW=105.99 g/mol) in the same manner. If 33.3 mL of the acid is required in the standardization process, what is the % protein in the serum? The protein is known to contain 16.0%N. (nitrogen AW=14.01 g/mol)

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