Questions 8-10, Answer the questions below based on information provided below:
You crossed the Hs-FLP; Act>CD2>Gal4, UAS-GFP flies with w; UAS-ArmS10 (activated fly β-catenin mutant) or w; UAS-Wg flies to induce ectopic Wg signaling activation in overexpression clones in wing discs after heat shock. Your lab partner used antibodies against DE-cadherin to determine the effect of ArmS10 or Wg expression. The results are shown in the images on the right (A-A” and B-B”).
- How do you identify cells that have ArmS10 or Wg overexpression? Please use arrows to point to the cells with ArmS10 or Wg overexpression. (1 point)
On the fluorescence microscopy images A, B, A” and ,B" have cells with GFP fluorescence indicating the presence of overexpressed ArmS10 or Wg.
- in the images A-A” on the right, is the effect of overexpression on DE-cadherin cell autonomous? What about in images B-B”?
In images A-A” DE-cadherin expression and localization changes are observed exclusively in GFP-positive cells (green cells), and not in the neighboring non-GFP cells, this suggests a cell-autonomous effect. In images B-B” DE-cadherin expression and localization changes are observed exclusively in GFP-positive cells (green cells), and not in the neighboring non-GFP cells, this suggests a cell-autonomous effect.
also if its possible, could you see if I answered this right?
- You lab partner want you to predict which panel (A-A” or B-B”) likely had ArmS10 expression and which one got Wg expression based on these
phenotypes. What are your predictions?
I need help with this one! ^^^^^^^^^^^^
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