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A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mM) Va (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. • y-int = 0.02217 • slope = [ Select 1 • Vmax= 10.40 v mM/min 1.18 • K = mM Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. • y-int = Select • slope = [ Select ] Vmax = [ Select ] mM/min [ Select ] mM

A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mM) Va (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. • y-int = 0.02217 • slope = [ Select 1 • Vmax= 10.40 v mM/min 1.18 • K = mM Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. • y-int = Select • slope = [ Select ] Vmax = [ Select ] mM/min [ Select ] mM

Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.9: Quality Assessment
Problem 1.1CT
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A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mM) Va (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. • y-int = 0.02217 • slope = [ Select 1 • Vmax= 10.40 v mM/min 1.18 • K = mM Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. • y-int = Select • slope = [ Select ] Vmax = [ Select ] mM/min • K. = [ Select ] mM ompotiti Inhibition
Transcribed Image Text:A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mM) Va (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. • y-int = 0.02217 • slope = [ Select 1 • Vmax= 10.40 v mM/min 1.18 • K = mM Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. • y-int = Select • slope = [ Select ] Vmax = [ Select ] mM/min • K. = [ Select ] mM ompotiti Inhibition
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