You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer of a 10-1 dilution and it has 38 colonies on it. Calculate the number of CFu/mL for this plate.

Q: when testing unpasteurized milk you get a colony count of 118 colonies on plate A and 110 colonies…

A: As the name suggest, the serial dilution is a series of the sequential dilution performed to convert…

Q: You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate…

A: Serial dilution is method of diluting a bacterial culture in succession. The process reduces the…

Q: If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original…

A: CFU stands for colony-forming unit that gives rise to a particular colony. It is generally assumed…

Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…

A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.

A: Introduction Coliform is the gram-negative, non-spore-forming and motile or non-motile bacterial…

Q: You do a serial dilution on an actively growing bacterial culture broth. Doing a plate count of your…

A: A serial dilution is the stepwise dilution of a substance in solution.  It is done to find out the…

Q: To decrease the unwanted biological damage for the patients during an x-ray test, we can: *

A: In laser production, increasing the density of the lasing medium will increase the number of photons…

Q: The number of bacteria in the samples need to be quantified. The laboratory uses a specific protocol…

A: Introduction :- Bacteria are single-celled organisms with a small size. Bacteria can be found…

Q: When one uses Benedict’s reagent added to UNKNOWN samples taken and added (as a liquid) by a pipette…

A: Sugars capable of reducing ferric or cupric ions are called reducing sugars. All monosaccharides…

Q: CITRATE UTILIZATION TEST WAS PERFORMED IN A CULTURE MEDIA WHICH CONTAIN A STRONG BUFFER. AND EVEN A…

A: In this test citrate agar is used, where citrate is carbon source and NH4and ability of organism to…

Q: A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate,…

A: Colony forming unit (CFU) is a unit that tells us the amount of viable cells present in the given…

Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…

A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…

Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

A: A colony-forming unit (cfu) is a unit used in microbiology to estimate the number of viable bacteria…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100…

A: Serial dilution is used to find the concentration of bacteria in the sample in case of greater…

Q: Kousei works at a company that produces sweetened condensed milk. If one or more samples exceed 10…

A: Cell count: The cell count data is generally utilized to predict the number of living microbes…

Q: For an In vitro mimicking infection test:

A: Introduction: Cell culture is the process of growing cells in a controlled setting, usually outside…

Q: In the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria…

A: Kirby-Bauer test: It is an antibiotic test also known as KB testing. It is used to test which…

Q: An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions.…

A: Number of colonies in the stock suspension = number of colonies plated * 1/ml* Dilution factor  here…

Q: You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the…

A:

Q: A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting…

A: Dilution : We know that the solute of the concentration will be decreasing in solution . There is a…

Q: Determine the number of bacteria per ml. of water specimen. 1ml 1ml 1ml water specimen H,0 blanks…

A:

Q: What is the purpose of the vial that does not contain chara?     (the solution with chara turned…

A: bromothymol blue is an acid base indicator. It is blue if the solution is alkaline and it become…

Q: You have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with…

A: The concentrated stocks can be diluted using the dilution formula. The dilution formula is:- C1V1 =…

Q: one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri…

A: Dilution factor = Volume final/volume initial  Df= 10/1 or 10 or 10¹ Number of colonies =200 Volume…

Q: A laboratory technician performed a series of 10 fold serial  dilutions shown below. he plated 0.1…

A: In this question, we are given colony count from different 10 fold dilutions. The volume plated from…

Q: With the constants you have for each objective, compute for the sizes of the given cells below.…

A: As we know that cell is the structural and functional unit of life it performing all the essential…

Q: 50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then…

A: Whenever a microrganism is plated for analytical purposes, it population is reported in terms of…

Q: At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing…

A: Homolysis or hemolytic cleavage is the cleavage of chemical bond usually in the presence of light or…

Q: A urine sample is diluted 1:1,000. If 0.1 ml were put into a Petri dish with melted agar, how  many…

A:

Q: You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent.…

A: The dilution factor can be defined as the ratio of Volume of the initial solution to the volume of…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial Dilution -- Serial dilution are used to calculate the concentration of microorganism .It…

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: Plating 0.1 mL of a sample diluted by a factor of 10^(-3) produced 43 colonies. What was the…

A: The question asks the original concentration (OCD) is to be identified in an undiluted…

Q: A cell population has 10,000,000 cells and you add a disinfectant. Assume the constant D-value is 5…

A: A cell is the basic structural and functional unit of an organism. A cell population includes a…

Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

A: Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the…

Q: Determine the number of bacteria per ml. of water specimen. 1ml 1ml 1ml water specimen H,0 blanks…

A: The aim of the serial dilution approach is to estimate the concentration of an unknown sample…

Q: If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40…

A: In the question it is asked that they want to plate out 40 bacteria, and this count comes under the…

Q: Which of the following is true Select one: O a. Inoculating loop and needle are transfer equipment…

A: Culture medium is the primary production environment for biological products. The pilot or scale-up…

Q: Observations and Results Average Count per ml of Sample (CFU/ml) Bacterial Dilution Factor ml of…

A: Introduction The Stepwise Dilution Of A Substance In Solution Is Referred To As Serial Dilution.…

Q: You dilute 1 mL of bacterial culture with 99 mL of water.  What is the resulting dilution factor?

A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…

Q: You are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What…

A: In this question, we are given with a solution of 1X concentration. We have to determine volume of…

Q: bacterial suspension with a population of 6.3 x 107cells/mL was diluted as follows: 4 mL was…

A: By using the Aseptic technique, The initial dilution is made 4mL into 16mL So, firstly dilution…

Q: If you had a cell suspension of 2.5 x 10^4 cells/mL and plated 100uL into each well (4 wells), how…

A: Given, total number of cells =2.5*104 cells/mL. 100 ul of this suspension is added to each well.

Q: a sample was diluted by placing a 0.1 mL of dilutent and 1 mL of this dilution was pour-plated.…

A: The CFU/ml in the Original Culture

Q: If you plated 0.1mL of a 103 dilution and you obtained 235 colonies on your agar plate, how many…

A: Introduction: CFU's: Colony-forming units are used to estimate the concentration of microorganisms…

Q: If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a…

A: Serial dilution  It is a method of stepwise dilution of a concentrated solution to get the desired…

Clinical Pharmacology

Pharmacology is a discipline of medicine that studies how medications interact with the systems and processes of living organisms, focusing on drug action mechanisms as well as therapeutic and other purposes.