You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells in a volume of 200 μL. Explain how you would do this.
You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells in a volume of 200 μL. Explain how you would do this.
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter24: New Technologies And Advances In Medical Instrumentation
Section: Chapter Questions
Problem 1Q
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Question
You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to
plate out 100 cells in a volume of 200 μL. Explain how you would do this.
Expert Solution
Step 1
We shall be performing a dilution calculation for this question.
We know, C1V1 = C2V2
- C1 is the concentration of the stock solution.
- V1 is the volume to be aliquoted from the stock.
- C2 is the final concentration of the diluted solution.
- V2 is the final volume of the diluted solution.
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