A chemist was able to extract a biologically active compound and wants to purify the sample since he is sure that the sample still contains impurities. After running the extract in TLC, the chemist found out that the sample produced only one spot near the solvent front. Suggest how the chemist can achieve a better resolution for the chromatogram.

Q: 8. A mixture of five alkyl iodides is separated by gas chromatography using a column packed with…

A: To find out the resolution of the following peaks.  (a) 2 and 3 (b) 4 and 5  (c) 5 and 6

Q: Identify The most commonly used stationary phase in paper chromatography.

A: The question is based on the concept of chromatography. It is a technique of separation and…

Q: How does the polarity of the mobile phase affect chromatograms?

A: Chromatograms are the pattern formed on an adsorbent medium by the layers of components separated by…

Q: explain what a response ratio is in Gas Chromatography and why it might be needed to quantify…

A: Gas chromatography (GC) is an analytical technique used to separate the chemical components of a…

Q: If you perform a column chromatography based on the present TLC analysis, which compound is eluted…

A:

Q: Metal ions chelate polar groups of analyte molecules for longer retention time. Does this affect…

A: HPLC is based on the principle where the sample (analyte) is distributed between a mobile phase…

Q: 1. Why do we use relative and not absolute retention times to characterize separated components?…

A: Retention time refers to the time that is spent by the solute in the stationary phase. The greater…

Q: 16 A 14E 12 10 4 10 20 30 40 Time, min 50 60 70 A chromatogram of a two-component mixture Detector…

A: Since we only answer up to 3 sub-parts, we will answer the first 3.Please resubmit the question and…

Q: What is the ideal TLC R; for column chromatography application? Why is it advisable to run a TLC…

A: The answer of the question is given below:

Q: Question attached

A: TLC It is abbreviated as thin layer chromatography. It is a technique used to isolate non-volatile…

Q: Is TLC chromatography and paper chromatography in a normal phase chromatography or reverse phase…

A: Solution- TLC- Thin layer chromatography. Paper chromatography

Q: errors in thin layer chromatography?

A: Thin Layer Chromatography (TLC) is used in order to separate components in a reaction mixture.

Q: Which one of the following approaches would be inappropriate for the introduction of the sample in…

A: ICP-MS is a type of mass spectroscopy which is used to ionize a sample.

Q: Explain the relationship between polarity of the compound and retention factor in chromatography

A: In chromatography, retention factor is the ratio of the distance travelled by the individual…

Q: Which of the spots traveled the furthest up the TLC plate corresponding to spot 3?

A: Given is, mobile solvent mixture of 95 % hexanes and 5 % ethyl acetate

Q: Is it possible to detect metal complexes of organic acids with thin layer chromatography?

A: Thin layer chromatography, abbreviated as TLC, is used to identify the element or compound present…

Q: A student researcher performed a chromatographic separation of caffeine and aspartame with methanol…

A: The retention time of methanol (tM), caffeine (tC) and aspartame (tA) is 34.9 s, 92.8 s and 153.5 s,…

Q: What are the principles involved in the separation of substances in Thin Layer Chromatography? 2.…

A: The question is based on the concept of organic reactions. We have to explain the principle of thin…

Q: Describe 4 similarities and 4 differences between Thin layer chromatography (TLC) and column…

A: The chromatography technique is used to isolate the components of mixtures that have two phases, one…

Q: a. Calculate the retention factor for each spot from lanes A to E . b. Which ink standard was the…

A: Paper Chromatography is a technique where paper is used as the stationary phase through which the…

Q: What types of columns are used in HPLC in chromatography?

A: HPLC stands for high performance liquid chromatography  which is used as a separation technique in…

Q: 2. Different marker inks were analyzed using a simple paper chromatography experiment. Two marker…

A: To calculate the retention factor of each spot from A to E.

Q: While preparing the mobile phase to run a monoamine neurochemical HPLC analysis, you miscalculated…

A: HPLC- High perfomance liquid chromatography is an advanced analytical technique used to identify and…

Q: 2. What are mobile and Stationary phases in a chromatogram? Site their nature.

A: The solution is given below -

Q: Which of the following is NOT a factor that affects GC retention time? Select an answer and submit.…

A: As per our guidelines, we are supposed to answer?️ only one question. Kindly repost other questions…

Q: What is Ultra-Performance Liquid Chromatography (UPLC)? What are the differences and advantages of…

A: The newest technology in liquid chromatography-based analysis is Ultra Performance Liquid…

Q: A student places an unknown sample on a thin-layer chromatography plate and develops it in…

A: The important points in the question are: An unknown sample was placed on a thin-layer…

Q: Differentiate between gel filtration and gel permeation chromatography.

A: Given : We have to tell what is the difference between gel filtration and gel permeation method.

Q: Explain how separation generally occurs in chromatography. What are the factors affecting the Rf…

A: Chromatography : Chromatography is a technique used in the laboratories for the separation of the…

Q: Explain the term gradient elution mode in HPLC Differentiate between gel filtration and gel…

A: The term elution mode in HPLC is defined as the separation method where the compounds are located…

Q: What is reverse-phase ion-pairing chromatography?

A: This is an more efficient Technique in which counter ions of solute particles are used .

Q: What are the main differences in column size, operating pressure, sample volume and run time between…

A: Chromatography is a technique  which is used for separating the components, or solute of a mixture…

Q: What is the difference between an ascending and descending paper chromatography?

A:

Q: Write down three differences between GC and HPLC (GC = gas chromatography) (HPLC =…

A: 1. High Performance Liquid Chromatography uses a liquid mobile phase and gas chromatography uses a…

Q: Which one of the following chromatographic techniques is useful in the identification of organic…

A: Chromatography is used to know the molecular weight . Here the known polymer is used to made the…

Q: What is the difference between normal-phase and reversed-phase chromatography?

A: Chromatography is a separation technique which has a mobile phase and a stationary phase and is used…

Q: An analysis of acid mixture was conducted using reversed-phase liquid chromatography with 20 mL/min…

A: Column length (L) = 10 cm Flow rate (r) = 20 mL/min Retention time of Y (tR,Y) = 4.4 min Width of Y…

Q: If retention times found on a chromatogram are 0.60 min for CH4 (=tm), 7.0 min for pentane, 8.0 min…

A: We use the formula below to find Kovat's retention index(I): Kovat's retention index, I = 100*n +…

Q: Paper Chromatography and TLC are examples of planar chromatography. True or FalsE?

A:

Q: What is the difference between a reconstructed total ion chromatogram and a selected ion…

A: Mass chromatography  is a technique used to analyze the structural features for given family of…

Q: 1. What would be the effect of the following errors in chromatographic work? a. Damaged silica gel…

A:

Q: Which among the following applies to reversed phase chromatography?    i. Compounds of low polarity…

A: The correct answer about reversed phase chromatography is given below

Q: Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result…

A: Thin-layer chromatography has two phases a stationary phase and a mobile phase. The silica gel…

Q: chromatogram below show a separation of a mixture of 2 components injected in a 25

A: The separation technique in which the liquid is a mobile phase where the molecules and sample ions…

Q: You were analyzing a mixture of volatile solvents using Gas Chromatography and found out that two…

A: Chromatography is the method for the separation of a mixture with very high resolution, which is an…

Q: enumerate the advantage of TLC methods of chromatography over column and paper chromatography.

A: Chromatography is a useful technique to precisely separate, analyse, and purify a wide range of…

Q: In a chromatographic analysis of an unknown mixture, compound A has a retention time of 5.56 minutes…

A:

Q: . What factors can affect a solute's Rf value in thin-layer chromatography? 2. Why is it necessary…

A: Answer - According to the question - Rf values and reproducibility can be affected by a number of…

Q: 1. Methanol and ethanol are separated in a capillary GC column with retention times of 370 and 385…

A: