(a) Consider the malate dehydrogenase reaction, which is part of the citricacid cycle:malate + NAD+ → oxaloacetate + NADH + H*In yeast mitochondria, where the pH = 8.1, this reaction is exergoniconly at low oxaloacetate concentrations. Assuming a pH = 8.1, a tem-perature of 37 °C, and the steady-state concentrations given below, cal-culate the maximum concentration of oxaloacetate at which the reactionwill still be exergonic.malate = 410 µMpyruvate = 3.22 mM lactate = 1.10 mMNAD+ = 20.0 mMΝADH -290 μΜCO, = 15.5 torrE°' (V)half reactionPyruvate + CO, + H* + 2ePyruvate + 2H+ + 2emalate-0.330lactate-0.190oxaloacetate + H*- pyruvate + CO2AG" = -30.9 kJ/mollactate + NAD*pyruvate + NADH + H*AG" = +25.1 kJ/mol(b) How would a drop in pH affect AG for the reaction described inpart (a)?(c) How would an increase in intracellular malate levels affect AG for thereaction in part (a)?(d) How would an increase in intracellular pyruvate levels affect AG for thereaction in part (a)?

Answered: Image /qna-images/answer/9b441c4f-a163-4fd8-8afc-35c046c619b2.jpg

Answered: (a) Consider the malate dehydrogenase…

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter11: Chemical Kinetics: Rates Of Reactions

Section: Chapter Questions

Problem 83QRT

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