A mutant form of polypeptide hormone has the following amino acid composition: Asp, Arg, lle, Met, Phe, Pro, Tyr, ValThe following observations are made:Trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest.Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro, and a hexapeptide containing all the others.Chymotrypsin cleaves the hormone into two tetrapeptides with the following composition: (Asp, Arg, Tyr, Val) and (lle, Met Phe, Pro)What is the amino acid sequence of this polypeptide?

In this biochemical analysis, we will investigate the amino acid composition and sequence of a…

Answered: A mutant form of polypeptide hormone…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A mutant form of polypeptide hormone has the following amino acid composition:

Asp, Arg, lle, Met, Phe, Pro, Tyr, Val

The following observations are made:

Trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest.
Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro, and a hexapeptide containing all the others.
Chymotrypsin cleaves the hormone into two tetrapeptides with the following composition: (Asp, Arg, Tyr, Val) and (lle, Met Phe, Pro)

What is the amino acid sequence of this polypeptide?

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