© Macmillan LearningYou need to prepare an acetate buffer of pH 5.54 from a 0.672 M acetic acid solution and a 2.90 M KOH solution. If youhave 780 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pl5.54? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.mL

To prepare an acetate buffer at pH 5.54 using acetic acid (CH3COOH) and potassium hydroxide (KOH),…

Answered: You need to prepare an acetate buffer…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

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What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.
can you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution § Prepare 1LProportions§ 0.25M tris§ 1.92M glycine§ pH 8.3§ 1% (w/v) SDSInstructions to prepare 100mL of 10X running buffer1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir todissolve.2. Check the pH with a calibrated pH metre once dissolved.§ The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5.3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution.4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.
You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.
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What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4
9. 3 μL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Substrate volume in mL Dilution factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL
200 microliters of a standard solution of 0.200 mg/mL caffeine was mixed with 4.8 mL of 50 mM sodium phosphate buffer, pH 3: methanol (60:40 v/v). What is the concentration of caffeine, in mg/mL, in this solution? Give your answer to two significant figures.
You need to make a protein buffer of: . • • 100 mM NaCl 25 mM Tris 8 5% w/v glycerol • 2 mM DTT Your stock solutions are: 5 M NaCl • 2 M Tris 8 • 70% w/v glycerol • DTT @ 154.25 g/mol How would you make a 1L protein buffer Solution? Show your work and describe.
10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944

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