A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in the nucleus. One way to resolve this question is to perform the following steps: • Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that are not expressing it. • Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled proteins. • Observe for fluorescence in the fused cell with two nuclei. c. Why is it important to stop the synthesis of more of the labeled proteins?
A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in the nucleus. One way to resolve this question is to perform the following steps: • Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that are not expressing it. • Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled proteins. • Observe for fluorescence in the fused cell with two nuclei. c. Why is it important to stop the synthesis of more of the labeled proteins?
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter10: Cell Division And Mitosis
Section: Chapter Questions
Problem 1ITD: Biologists have long been interested in the effects of radiation on cells. In one experiment,...
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ANSWER QUESTION C and EXPLAIN
A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this
is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in
the nucleus. One way to resolve this question is to perform the following steps:
• Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that
are not expressing it.
• Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled
proteins.
• Observe for fluorescence in the fused cell with two nuclei.
c. Why is it important to stop the synthesis of more of the labeled proteins?
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