A scientist wants to create a new enzyme for the synthesis of a new small molecule that will be used in the treatment of lung cancer. The scientist wants to use a technique that allows to shuffle protein domains. Protein A and Protein B are 5 and 7 different domains respectively. If 3 domains from A and 4 domains from B are chosen and paired. 1- How many possible arrangements are present? 2-How many primers are needed to amplify domain sequences to prepare all constructs?

A scientist wants to create a new enzyme for the synthesis of a new small molecule that will be used in the treatment of lung cancer. The scientist wants to use a technique that allows to shuffle protein domains. Protein A and Protein B are 5 and 7 different domains respectively. If 3 domains from A and 4 domains from B are chosen and paired. 1- How many possible arrangements are present? 2-How many primers are needed to amplify domain sequences to prepare all constructs?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter11: Genome Alterations: Mutation And Epigenetics

Section: Chapter Questions

Problem 10QP: If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense...

See similar textbooks

Similar questions

A scientist wants to use a technique that allows to shuffle protein domains. Protein A and Protein B are 5 and 7 different domains respectively. If 3 domains from A and 4 domains from B are chosen and paired how many primers are needed to amplify domain sequences to prepare all constructs?
How might a single base substitution in the sequence of a gene affect the amino acid sequence of a protein encoded by the gene, and why? A - It is not possible for a single base substitution to affect protein structure, because each codon is three bases long. B- The amino acid sequence would be substantially altered, because the reading frame would change with a single base substitution. C - Only a single amino acid could change, because the reading frame is unaffected. D- All amino acids following the substitution would be affected, because the reading frame would be shifted.
You are a molecular biologist studying a defective human protein that is known to be a cause of a rare cancer. You isolate the suspected protein from a person with this cancer and compare it to the same protein in a person without the cancer. You discover that the protein isolated from the cancer patient is slightly smaller in size when compared to the protein from the unaffected patient. Furthermore, you discover that the protein from the cancer patient differs in almost every amino acid starting after the 23rd amino acid. What is the likely explanation?
In the image below, the blue squiggly line represents a folded protein. There are two particular amino acids that are colored red and black in the protein. The structure of each of these two specific amino acids is shown in the indicated box. A mutation occurs in the gene that encodes the protein illustrated above. The result of this mutation is that one of the amino acids above is substituted with another. Specifically, the amino acid whose structure is shaded red above (the one on the left in the illustration) is replaced by the amino acid shown below. Use this information to answer the two questions that follow. Please answer these questions. 1. Based on this description, indicate which type of mutation occurred: missense, nonsense, silent, or indel 2. How would this mutation affect this protein's structure and function? Explain in 2-4 sentences. Be specific, and be sure to include details of these specific amino acids in your response. (I'm giving you the…
You are a molecular biologist studying a defective human protein that is known to be the cause of a rare cancer. You isolate the suspected protein from a person with this cancer and compare it to the same protein in a person without the cancer. You discover that the protein isolated from the cancer patient is slightly smaller in size when compared to the protein from the unaffected patient. Furthermore, you discover that the protein from the cancer patient differs in almost every amino acid starting after the twenty-third amino acid. What is the likely explanation?
A small section of a gene for a protein has the following nucleotide sequence: CCT AAG GAT TCA CTT Which of the following mutations would cause a missense mutation in the sequence shown above? a. Replacement of first guanine base with cytosine base b. Replacement of first thymine base with cytosine base c. Replacement of second thymine base with adenine base d. Replacement of seond guanine base with adenine base
A small section of a gene for a protein has the following nucleotide sequence:CTG GGA TCC TAA GGTWhich of the following mutations would cause a nonsense mutation in the sequence shown above? Select one: a. Replacement of second adenine base with a cytosine base b. Insertion of guanine base after the first thymine base c. Insertion of guanine base after the first adenine base d. Replacement of second cytosine base with a adenine base
In a particular region of the genome of a certain bacterium, one DNA strand is transcribed to give rise to an mRNA for protein A and the other DNA strand is transcribed to give rise to an mRNA for protein B. a) Would there be any problem in expressing the two genes? 2. b) If a mutation occurred to affect the structure of protein A, what would you observe in the structure of protein B?
What will be the order of amino acids derived from the following DNA sequence 5’-TGATCGCACAAT-3’? Explain briefly. (1.5) If the base G (denoted by an asterisk) in the sequence 5’-TGATCG*CACAAT-3’ is replaced by C due to a mutation, the new sequence will be 5’-TGATCCCACAAT-3’ what will be the new amino acid sequence? Explain briefly. (1.5) If the anticodon sequence of a tRNA is 5’-GCG-3’, what amino acid will it carry? Explain briefly. (1.5) What would be the effect of mutation if the C is changed to A in the anticodon? Explain briefly. (1.5)
A small section of a gene for a protein has the following nucleotide sequence: GCT CTA GCT ATC TGA Which of the following mutations would cuase a silent mutation in the sequence shown above? a. Replacement of second adenine base with thymine base b. Replacement of first thymine base with adenine base c. Replacement of second guanine base with cytosine base d. Replacement of first cytosine base with guanine base
By average, how many Sau3A (5’GATC3’) sites are there in a 10 kd DNA molecule? (1/4)^6 * 10,000 = 2.44140625 => 2.4 For the above 10 kb DNA, if you use Sau3A to do a complete digestion, you will only get a lot of small fragments ( 200~500 base pairs). What should I do if I want to get larger digested fragments (say 3 to 5 kb) Please answer asap and type your answer and do not copy from anywhere please
a molecular geneticist hopes to find a gene in human liver cells that codes for an important blood-clotting protein. he knows that the nucleotide sequence of a small part of the gene is gtggactgaca. briefly explain how to obtain the desired gene answer