Asked Jan 29, 2020

3. One gram of soil was suspended in 9 ml saline and thoroughly mixed. From this suspension, 4 serial, 1:10 dilutions were made. 200 hundred microliters of each of the last four serial dilutions were used for spread plating on TY media, resulting in 5600 colonies, 412 colonies, 54 colonies and 8 colonies. What do you calculate as the bacterial concentration in the soil? (For your information 1 gram = 1 ml)


Expert Answer

Step 1

CFU, colony-forming unit, is a unit that is used to determine the number of viable fungi or bacteria in the given sample. Microbe culturing and viable cells are required to count by CFU. Generally, one viable cell can give rise to a complete colony by the process of replication or cell division. Moreover, bacteria generally form a colony in the form of chains or clumps.

Step 2

The bacterial concentration in the soil is calculated by the below formula:

Biology homework question answer, step 2, image 1
Step 3
Biology homework question answer, step 3, image 1

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