A standard 1 mL cuvette is three-quarters filled with a 1 in 6 dilution of 24 μmol.L-1 oxyhaemoglobin. The absorbance is measured at 600 nm. A few drops of a live yeast suspension is added to the cuvette (without significantly changing the volume). The absorbance is remeasured at the same wavelength as above and is found to be 0.60, a decrease of 0.35 on the first measurement. What is the molar absorbance coefficient of the deoxyhaemoglobin at 600 nm? (do not enter the units in the answer, 2 decimal places)
A standard 1 mL cuvette is three-quarters filled with a 1 in 6 dilution of 24 μmol.L-1 oxyhaemoglobin. The absorbance is measured at 600 nm. A few drops of a live yeast suspension is added to the cuvette (without significantly changing the volume). The absorbance is remeasured at the same wavelength as above and is found to be 0.60, a decrease of 0.35 on the first measurement. What is the molar absorbance coefficient of the deoxyhaemoglobin at 600 nm? (do not enter the units in the answer, 2 decimal places)
Chapter26: Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 26.1QAP
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A standard 1 mL cuvette is three-quarters filled with a 1 in 6 dilution of 24 μmol.L-1 oxyhaemoglobin. The absorbance is measured at 600 nm. A few drops of a live yeast suspension is added to the cuvette (without significantly changing the volume). The absorbance is remeasured at the same wavelength as above and is found to be 0.60, a decrease of 0.35 on the first measurement.
What is the molar absorbance coefficient of the deoxyhaemoglobin at 600 nm? (do not enter the units in the answer, 2 decimal places)
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