A stock solution of alcohol dehydrogenase (ADH) has been diluted 1 in 10 and the absorbance reading at 280 nm of this diluted solution is 0.04. Given that the absorption coefficient for ADH at 280 nm is e mg/ml = 1.6 and that you require to do 30 assays in triplicate, each assay using 2 ug of enzyme, how many ul of the stock ADH do you need to do the experiment?

A stock solution of alcohol dehydrogenase (ADH) has been diluted 1 in 10 and the absorbance reading at 280 nm of this diluted solution is 0.04. Given that the absorption coefficient for ADH at 280 nm is e mg/ml = 1.6 and that you require to do 30 assays in triplicate, each assay using 2 ug of enzyme, how many ul of the stock ADH do you need to do the experiment?

Organic Chemistry

8th Edition

ISBN:9781305580350

Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions

Section: Chapter Questions

Problem 20.26P

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