QUESTION 1 You obtained the following raw data when setting up a Bluret standard curve BSA (mgim) Absorbancy 540nm 0158 0210 0260 3 0305 4. 0360 0.410 0455 7. 0510 0530 0550 10 0554 Ater blanking against a biuret d0 sample. the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0324 was obtained Set up a standard curve excluding outiers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures)

QUESTION 1 You obtained the following raw data when setting up a Bluret standard curve BSA (mgim) Absorbancy 540nm 0158 0210 0260 3 0305 4. 0360 0.410 0455 7. 0510 0530 0550 10 0554 Ater blanking against a biuret d0 sample. the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0324 was obtained Set up a standard curve excluding outiers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

A protein consists of two types of peptide chains (A and B) with an unknown stoichiometry (AxBy). When you ran this protein directly on reversed phase-HPLC with UV monitor set at 280 nm, two peaks were resolved. Mass spec determined that the peaks represented Chain A and Chain B, respectively. The peak area is 500,000 for Peak A (Chain A) and 100,000 for Peak B (Chain B). The molecular masses of Chain A and Chain B are 25,000 and 5000, respectively. The extinction coefficients for Chain A and Chain B are 1 mL/mg.cm and 0.5 mL/mg.cm, respectively. Please calculate x/y.
q41 IF YOU DO NOT KNOW THE ANSWER PLEASE DO NOT REJECT IT please calculate the unknown concentration of the protein D wih an absorbance value of A412 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762
You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
By using the following mentioned concentrations and their corresponding absorbance values prepare a standard curve in MS-EXCEL and write down the CURVE equation along with R2 value. Next calculate the concentrations of proteins of two unknown samples from that obtained equation. The absorbance of the unknown samples are: 0.375and 0.197.
Question in picture. Ksp of SrF2 = 4.3x10^-9
The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (μg) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 a. use excel, another program, or graph paper to produce a calibration curve based on the data. b. Use linear regression (best fit line) to obtain the slope of the line c. Determine the unknown protein content for a sample that shows an absorbance reading of 0.150.
What are analytes and how can they be used for biochemical recognition?
Explain and elaborate. Show solution and sketch if possible. No plagiarism.
Explain it with hand written answer
plz explain and give correct and typed answer
Hydrocarbons in the cab of an automobile were measured during trips on the New Jersey Turnpike and trips through the Lincoln Tunnel connecting New York and New Jersey. The total concentrations ( ± standard deviation) of m- and p-xylene wereTurnpike: 31.4 ± 27.0 μg/m3 (32 measurements)Tunnel: 54.9 ± 29.8 μg/m3 (32 measurements)Do these results different at the 95% confidence level?
Q3) A solution of Tryptophan has an absorbance at 280 nm of 0.25 in a 0.5 cm length cuvette. Given the absorbance coefficient of Tryptophan is 6.4 × 103 L Mol-1 cm-1. What is the concentration of solution?