In 250 ml ethanol solution, a tablet of saccharin was dissolved, and it was measured by UV spectrometer at a wavelength of 310 nm. The absorption was 0.45, the cell length was 1 cm, and the molar absorptivity was 12500. Calculate: the concentration of saccharin in the solution?
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- As part of an investigation on Pb in biological ecosystems, some grasses that grew along the road and were exposed to emissions from motor gasoline were collected. 6.2501 g of grass were calcined to destroy all organic matter, the inorganic residue was properly treated and finally made up to 100 mL. An aliquot of 50 mL of this solution was read in an atomic absorption spectrophotometer registering an absorbance of 0.1250; then 0.10 mL of a standard solution of 55 µg of Pb / mL were added to the other 50 mL, with this fortified sample, another absorbance measurement was made, which was 0.1798. Calculate the µg of Pb / g of grasschemhelp7 The researcher determined the Pb content of water sample from a river suspected to be contaminated with Pb from a nearby factory by complexing with cyanidine and the absorbance measured was 0.405. What is the lead content of the water sample? a. 4.23 ppm b. 4.28 ppm c. 207 ppm d. 333 ppmIf the absorbance of 0.291 M copper (II) chloride solution is 0.497 and the path length of light is 1.00 cm, what is the Beers Law extinction coefficient?
- What is the pathlength in centimeters when the molar absorptivity for a given absorbing species is 1.32 ¥ 103 L mol-1 cm-1, the concentration is 0.000923 M, and the absorbance is 0.493?You did not allow enough time for the solution to reach equilibrium before measuring its absorbance. Explain how this will affect the reported value of Kc.A solution of tryptophan has an absorbance of 0.64 at 280 nm. Given with of 6.04 x 103 Msolution of tryptophan and path length of 0.5 cm. What is the molar absorptivity oftryptophan?
- Mercury(II) forms a 1:1 complex with triphenyltetrazolium chloride that exhibits an absorption maximum at 255 nm. The mercury(II) in a soil sample was extracted into an organic solvent containing an excess of TTC , and the resulting solution was diluted to 100.0 mL in a volumetric flask. Five-milliliter aliquots of the analyte solution were then transferred to six 25-mL volumetric flasks. A standard solution was then prepared that was 5*10^-6 M in . Volumes of the standard solution shown in the table were then pipetted into the volumetric flasks, and each solution was then diluted to 25.00 mL. The absorbance of each solution was measured at 255 nm in 1.00-cm quartz cells. Please solve and explain these questions: A.) Enter the given data pictured above into a spreadsheet and show the correct standard additions plot B.) Determine the slope and the intercept of the line C.)Determine the standard deviation of the slope and the intercept. D.) Calculate the concentration of Hg(II) in the…A substance is known to have a molar extinction coefficient of 18,300mlxcm-1xmg-1 at its wavelength of maximum absorption. The absorption of a solution containing this substance in a 1-cm sample cell was found to be 0.327 at this same wavelength. Determine the concentration of the solution.Experiment: UV-Vis Spectrophotometry and Beer’s Law Absorption of Light of Dyes in Beverages A calibration curve for the orange dye in a soft drink was obtained by plotting absorbance against concentration (M) for a series of solutions of known concentration. The slope of the calibration curve was 226 L/mol and the intercept was -0.011. The absorbance of an unknown soft drink was found to be 0.333 under the same conditions as the calibration curve. Calculate the concentration of the orange dye in the soft drink.
- Pd(II) and Au(II) can be determined simultaneously by the reaction with methiomeprazine (C 19 H 24 N 2 S 2 ). The maximum absorption for Pd occurs at 480 nm, while for the Au complex it is at 635 nm. A sample of 25 mL was treated with excess of the complexant and then diluted to 50.0 mL. Calculate the molar concentrations of Pd(II) and Au(II) in the sample. The data are found in the table below and the bucket used was 1.0 cm.Which of the following is not needed to use uv-vis absorption spectrometry as a quantitative technique ? a. The sample has to be in a solution form. b. The sample does not contain other molecules that absorb in wavelength used for analysis. c. The compound that will be monitored obey’s Beer Lambert’s law. d. The compound that will be monitored has only one Lambda max (wavelength with maximum absorption).A 2.0 gram of a certain substance with a molecular weight of 100 g/mol is dissolved in a 500 ml solution. The length of the cuvette is 2.0 cm and absorbance of 0.301. What is the extinction coefficient in cm-1 M-1?